文献类型：期刊文献

英文题名：One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli

作者：Lai, Ningyu[1];Luo, Yuanchan[1];Fei, Peng[1];Hu, Peng[4];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Shanghai GTL Biotech Co Ltd, 1688 North Guoquan Rd, Shanghai 200438, Peoples R China

年份：2021

卷号：6

期号：3

起止页码：144

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000702747200002)】；

基金：We thank Professor Shu Quan for kindly providing us with the strain, E. coli BL27. This study was supported by the Natural Science Foundation of Shanghai (19ZR1472700), the Fok Ying-Tong Education Foundation, China (Grant No. 161017), the National Natural Science Foundation of China (Grant No. 21776083), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014). Partially supported by Open Funding Project of the CAS Key Laboratory of Synthetic Biology.

语种：英文

外文关键词：Syngas-derived acetic acid; 3-Hydroxypropionic acid; Metabolic engineering; Escherichia coli; Dynamic regulation

摘要：Syngas, which contains large amount of CO2 as well as H-2 and CO, can be convert to acetic acid chemically or biologically. Nowadays, acetic acid become a cost-effective nonfood-based carbon source for value-added biochemical production. In this study, acetic acid and CO2 were used as substrates for the biosynthesis of 3-hydroxypropionic acid (3-HP) in metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus. Strategies of metabolic engineering included promoting glyoxylate shunt pathway, inhibiting fatty acid synthesis, dynamic regulating of TCA cycle, and enhancing the assimilation of acetic acid. The engineered strain LNY07(M*DA) accumulated 15.8 g/L of 3-HP with the yield of 0.71 g/g in 48 h by wholecell biocatalysis. Then, syngas-derived acetic acid was used as substrate instead of pure acetic acid. The concentration of 3-HP reached 11.2 g/L with the yield of 0.55 g/g in LNY07(M*DA). The results could potentially contribute to the future development of an industrial bioprocess of 3-HP production from syngas-derived acetic acid.