文献类型：期刊文献

中文题名：施氏假单胞菌应用于IPS技术的可行性试验研究

英文题名：Feasibility experiment on using pseudomonas stutzeri in IPS

作者：彭尔兴[1,2,3];章定文[1,2,3];周利[4];孙鹏程[4]

机构：[1]东南大学交通学院,南京210096;[2]东南大学江苏省城市地下工程与环境安全重点实验室,南京210096;[3]南京水利科学研究院水利部土石坝破坏机理与防控技术重点实验室,南京210029;[4]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2017

卷号：47

期号：1

起止页码：170

中文期刊名：东南大学学报（自然科学版）

外文期刊名：Journal of Southeast University：Natural Science Edition

收录：CSTPCD;;EI(收录号：20171003422141);Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：水利部土石坝破坏机理与防控技术重点实验室开放研究基金资助项目(YK914021);中央高校基本科研业务费专项资金资助项目(2242014R30020);江苏高校"青蓝工程"优秀青年骨干教师培养对象资助项目;江苏省创新计划资助项目(KYLX15_0158)

语种：中文

中文关键词：IPS;施氏假单胞菌;反硝化作用;温度;pH值

外文关键词：induced partial saturation (IPS) ; pseudomonas stutzeri; denitrification; temperature; pH

摘要：为探究利用施氏假单胞菌代谢产物——氮气加固可液化砂土的可行性,对该菌反硝化作用的条件与砂样中产气效能进行了研究,分析了不同碳源对NO_3^--N与NO-2-N还原率的影响以及温度和初始p H值对其最终饱和度、平均产气速率、初始停滞期的影响,并确定了最优碳源、适用温度和初始p H值区间.试验结果表明,柠檬酸钠为最优碳源,该菌在21 h时对NO_3^--N还原率达到100%,NO-2-N存在先累积后还原的过程,并伴有0.82 mmol/L残留浓度.该菌在4~30℃下均可产气.随温度升高,平均产气速率大幅增加,砂样最终饱和度略有降低.恒温20℃且初始p H值为5~9时,该菌可在砂样中顺利产气.中性及碱性环境下砂样的最终饱和度基本一致,酸性环境下砂样的最终饱和度随初始p H值的减小而降低,细菌在砂样中的平均产气速率随初始p H值的下降而减小.与已有微生物气法相比,将该菌应用于IPS技术中具有平均产气速率快、初始停滞期短、工艺简单等优点.
To explore the feasibility of the reinforcement of liquefiable sand by nitrogen,the metabolitesof pseudomonas stutzeri,the denitrification condition and the gas production efficiency of soilare studied.The influences of different carbon sources on the reduction rates of N03^-N and N02^-N as well as the impacts of the temperature and the initial pH value on the final saturation,the average rate of gas production,the initial stagnation period are analyzed.The optimum nitrogen source,the suitable ranges of the temperature and the initial pH value are determined.The experimental results show that Na-citrate is the optimum nitrogen source.The reduction rate of N03^-N can reach100%at21h.N02^-N accumulates first before reduction with0.82mmol/L residual.The bacteria has the ability to generate gas when temperature is from4to30°C.With the increase of the temperature,the average rate of gas production increases significantly and the final saturation of the sand simple decreases a little.When the temperature is20°C and the initial pH value is5to9,the bacteria can smoothly generate gas in the sand simple.In the neutral and alkaline environments,the final saturations of the sand simple are basically consistent.In the acidic environment,the final saturation of the sand simple decreases with the decrease of the initial pH value.The average rate of gas production of the bacteria in the sand simple decreases with the decrease of the initial pH value.Compared with the existing biogas methods,the application of this bacteria in induced partial saturation(IPS)has advantages such as the fast average rate of gas production,short initial stagnation period,simple technologyand so on.