文献类型：期刊文献

英文题名：Low-Bleed Silica-Based Stationary Phase for Hydrophilic Interaction Liquid Chromatography

作者：Qian, Kun[1];Yang, Zhanqiang[1];Zhang, Feifang[1];Yang, Bingcheng[1];Dasgupta, Purnendu K.[2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA

年份：2018

卷号：90

期号：15

起止页码：8750

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20182905557843);WOS:【SCI-EXPANDED(收录号:WOS:000441476600007)】；

基金：This work was sponsored by the National Natural Science Foundation of China (No. 21477037, 21322502). B.C.Y. gratefully acknowledges support from the Fundamental Research Funds for the Central Universities (22221818014). P.K.D. acknowledges the US National Science Foundation for enabling his participation (CHE-1506572).

语种：英文

外文关键词：Hydrophobicity - Liquid chromatography - Coatings - Effluents - Hydrophilicity - Polyvinyl alcohols

摘要：Bleeding of hydrophilic interaction liquid chromatographic (HILIC) phases is a major problem. A hydrophobic silica surface exhibits low bleeding but does not behave as a HILIC phase. A hydrophilic coating, such as that of poly(vinyl alcohol) (PVA) on a hydrophobic particle should result in a low-bleed hydrophilic phase. However, a silica particle functionalized in a hydrophobic manner is not wetted by PVA and cannot be coated with it. Coating with PVA becomes possible if one region of the hydrophobic functionality is polar. We describe a low-bleed HILIC stationary phase, PVA-coated benzylthioethyl-silica. The benzyl groups shield the silica from water erosion, while the thiol group provides sufficient local polarity for PVA to wet the material. The new stationary phase demonstrated good chromatographic performance and typical HILIC retention behavior. The measured silica concentration in the effluent was similar to 80-fold lower than that from a bare silica column. The new stationary phase exhibited a lower background level, lower background noise, and lower background drift under gradient conditions than benchmark commercial columns.