文献类型：期刊文献

英文题名：Hyperosmotic Adaptation ofPseudomonas protegensSN15-2 Helps Cells to Survive at Lethal Temperatures

作者：Wang, Xiaobing[1];Tang, Danyan[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：25

期号：3

起止页码：403

外文期刊名：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

收录：;EI(收录号：20202708884966);WOS:【SCI-EXPANDED(收录号:WOS:000544818100006)】；

语种：英文

外文关键词：Pseudomonas protegens; transcriptomic; hyperosmotic; stress resistance

摘要：Pseudomonas protegensis a gram-negative bacterium with an excellent biological control effect. Compared to standard cells growing in NaCl-free media, the ability of the hyperosmotic cells (450 mM NaCl) to resist high temperatures and freezing was significantly improved. It is of great significance to apply theP. protegensto elaborate on the hyperosmotic adaptation mechanism. RNA-seq was used to sequenceP. protegenscultured with 0 mM and 450 mM NaCl. Comparative transcriptomic analyses of the different treatments were performed using gene ontology and the Kyoto encyclopedia of genes and genome. The results revealed that hyperosmotic stress had prominent impacts on the genes involving in multiple cellular functions. The hyperosmotic environment significantly affected carbohydrate, energy, and amino acid metabolism, as well as membrane system and cell motility. Our findings indicated thatP. protegensadopted a series of approaches, including the high cytoplasmic concentrations of potassium ions and the uptake or synthesis of osmoprotectants, for surviving hyperosmotic stress. Among these, trehalose and proline synthesis appeared to be an important method to withstand prolonged hyperosmotic stress inP. protegens.These data provide crucial resource that may determine specific responses to the hyperosmotic environment inP. protegens.