文献类型：期刊文献

英文题名：A PEG-Lysozyme hydrogel harvests multiple functions as a fit-to-shape tissue sealant for internal-use of body

作者：Tan, Haoqi[1];Jin, Dawei[2];Qu, Xue[1];Liu, Huan[1];Chen, Xin[1];Yin, Meng[2];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Cardiothorac Surg, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China

年份：2019

卷号：192

起止页码：392

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20190106332457);WOS:【SCI-EXPANDED(收录号:WOS:000456902000032)】；

基金：This study was supported by National Natural Science Foundation of China (31330028, 51621002, 51573047), the 111 project (B14018), the science and technology innovation project of Shanghai Science and Technique Committee (18441908300), Collaborative Innovation Center for Translational Medicine at Shanghai Jiao Tong University School of Medicine (TM201504), the Open Research Fund of State Key Laboratory Polymer Molecular Engineering (Fudan University) (K2017-23).

语种：英文

外文关键词：Lysozyme; 4-Arm-Poly ethylene glycol; Injectable hydrogel; Tissue sealing; Cell attachment; Antimicrobial

摘要：In situ formation of surgical sealants to stop internal fluids leakage is more attractive compared to the traditional suture or staple. However, commercial sealants have weak points in tissue adhesive, cell affinity, antibacterial etc., which make them remain suboptimal for internal use of body. It is required to develop multifunctional sealants that can meet clinical needs. Herein, a PEG-lysozyme (LZM) injectable sealant composed of 4-arm-PEG and lysozyme was developed. Lysozyme offers free amine groups to rapidly cross link with PEG. The hydrogel can tightly adhere to tissues and provide good mechanics to withstand high pressure. Moreover, lysozyme innately confers antibacterial and cell affinity on the hydrogel that are usually lacking in marketed sealants. The hydrogel is easily operated to seal gas or blood leakage in a rabbit trachea and artery defect. Moreover, it can close the transmural left ventricular wall defect on a beating heart. The traumatic organ functions completely recovered postoperatively. Considering the good biocompatibility and the simple fabrication process, the PEG-LZM hydrogel is promising to clinical transformation. More broadly, our work indicates that nature-occurring molecules are versatile building blocks for construction of materials and confer functions, which represents a simple tragedy to develop advanced functional biomaterials.