文献类型：期刊文献

英文题名：Ratiometric and reusable fluorescent nanoparticles for Zn2+ and H2PO4- detection in aqueous solution and living cells

作者：He, Chunsheng[1]; Zhu, Weiping[1]; Xu, Yufang[1]; Zhong, Ye[1]; Zhou, Juan[1]; Qian, Xuhong[1]

机构：[1] Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2010

卷号：20

期号：47

起止页码：10755

外文期刊名：Journal of Materials Chemistry

收录：EI(收录号：20104813434783)

语种：英文

外文关键词：Chromophores - Computer software reusability - Dynamic light scattering - Spectroscopic analysis - High resolution transmission electron microscopy - Silica nanoparticles - Shells (structures) - Scanning electron microscopy - Fluorescence

摘要：In this work, three kinds of core-shell silica nanoparticle-based fluorescent materials were prepared based on a modified St?ber-Van Blaaderen method. They were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), dynamic light scattering (DLS), FT-IR, and several other spectroscopic methods. Firstly, The silica@sensor-1 nanoparticle (SSN) showed high selectivity toward Zn2+, which can detect Zn2+ in aqueous solution and living cells. It also can be reused to detect Zn2+ for at least four cycles after a simple regeneration. Secondly, to create a ratiometric measurement platform, the dye-2@silica nanoparticles (DSN), a new class of core-shell fluorescent silica nanoparticles were prepared with an acenaphtho[1, 2-b]pyrrol-9-carbonitrile chromophore derivative as the inner reference. It showed negligible sensing properties towards Zn2+, and the fluorescent intensity was not subjected to interference induced by pH change. Thirdly, the dye-2@silica@sensor-1 nanoparticles (DSSN), with the above reference dye buried inside the silica matrix and a layer of chemosensors anchored onto the surface of silica nanoparticles were prepared. DSSN showed not only the same sensing ability as SSN, but also a clear ratiometric fluorescent signal toward Zn 2+ in aqueous solutions and living cells. On the other hand, H 2PO4- is a well-known Zn2+ binder, so the [DSSN@Zn2+] complex was found to ratiometrically detect H 2PO4-. It responded to H2PO 4- at a neutral aqueous solution with a detection limit lower than 6 × 10-6 M. Moreover, the ratio of fluorescence intensity was linearly increased in the range 6～500 μM of H 2PO4-, which implies a potential application for the quantitation of H2PO4- in aqueous solution. To the best of our knowledge, this is the first example of core-shell silica nanoparticle-based fluorescent materials that can be repeatedly used to ratiometrically detect Zn2+ and H2PO4- in 100% neutral aqueous solutions. ? 2010 The Royal Society of Chemistry.