文献类型：期刊文献

英文题名：Production of 3-Hydroxypropionic Acid from Syngas-Derived Acetic Acid by Engineered Vibrio natriegens Combined with Adaptive Laboratory Evolution

作者：Xu, Mingcheng[1];Luo, Yuanchan[1];Liu, Xiaozhen[1];Fei, Peng[1];Lu, Juefeng[1];Wang, Yuying[1];Wu, Hui[1,2,3,4]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[4]China Natl Light Ind Council, Key Lab Biobased Mat Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：11

期号：10

起止页码：4125

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20230913655865);WOS:【SCI-EXPANDED(收录号:WOS:000950331000001)】；

基金：ACKNOWLEDGMENTS This work was supported by the National Key R&D Program of China (2021YFC2103500) and Science and Technology Commission of Shanghai Municipality (21DZ1209100) and partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：acetic acid; 3-hydroxypropionic acid; Vibrio natriegens; metabolic engineering; metabolic evolution

摘要：Vibrio natriegens is a more promising alternative chassis microorganism for the biotechnological process due to its extremely fast growth rate and the ability for efficient and rapid utilization of a variety of industrial carbon sources. Here, we developed engineered V. natriegens to produce 3-hydroxypropionic acid (3-HP). The biosynthesis of 3-HP from refined or syngas-derived acetic acid is achieved by the introduction of the heterogeneous malonyl-CoA pathway, optimization of fermenta-tion conditions, adaptive laboratory evolution for enhancing the toxic tolerance to the substrate, and whole-cell biocatalysis. The engineered strain could efficiently accumulate 3-HP with a titer of 3.78 g/L yielding 0.25 g/g within 24 h. In addition, 2.2 g/L 3-HP was obtained from syngas-derived acetic acid with a yield of 0.15 g/g. This study provided a novel strategy for producing 3-HP from syngas-derived acetic acid with engineered V. natriegens.