文献类型：期刊文献

中文题名：放线菌SSPRC-1活性代谢产物的分离鉴定

英文题名：Isolation and identification of bioactive metabolites from Actinomycetes SSPRC-1

作者：王雪啸[1];宗蜜蜜[1];徐文平[1];程亮[1];陶黎明[1]

机构：[1]华东理工大学药学院,上海市化学生物学(芳香杂环)重点实验室,上海200237

年份：2016

卷号：41

期号：12

起止页码：898

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：“十二五”国家科技支撑项目绿色生态农药的研发与产业化(No.2011BAE06B04)

语种：中文

中文关键词：放线菌;分离纯化;结构鉴定;生物活性

外文关键词：Actinomycetes sp.;Isolation and purification;Structure elucidation;Bioactivity

摘要：目的对土壤放线菌SSPRC-1次级代谢产物中杀虫和抑菌活性物质进行研究。方法对发酵液通过正丁醇萃取、硅胶柱色谱、HPLC等手段进行分离纯化,利用质谱、核磁共振谱等现代波谱分析方法鉴定活性化合物结构。结果从放线菌SSPRC-1发酵液中分离得到3个活性化合物SSPRC-1-1、SSPRC-1-2和SSPRC-1-3,确定SSPRC-1-1分子式为C15H10O4,是异黄酮类物质,在天然产物中未曾发现,仅有文献报道可通过化学合成得到,暂命名为isoflavones H;SSPRC-1-2分子式为C15H10O5,与异黄酮类物质三羟基异黄酮genistein相同;SSPRC-1-3分子式为C25H37NO4,与杀粉蝶毒素A结构相同。结论化合物SSPRC-1-1和SSPRC-1-2对Pyricularia oryzae的MIC分别为125和24μg/m L,化合物SSPRC-1-3对黏虫具有较好的毒杀作用。
Objective To investigate the activities of the secondary metabolites against Pyricularia oryzae and Mythimna separate(Walker)from the strain Streptomyces SSPRC-1.Methods By bioassay-guided isolation and purification,the compounds were isolated and purified from fermentation with n-butyl,silica gel column chromatography and HPLC,and their structures were elucidated with the help of the spectra data analysis of UV,ESI-MS and NMR.Results Three compounds were isolated from the fermentation broth of Streptomyces SSPRC-1 and named as SSPRC-1-1,SSPRC-1-2 and SSPRC-1-3.The molecular formula of the compound SSPRC-1-1 was confirmed as C15H1004,which was identified as a new natural compound named isoflavone H.The molecular formulas of the compound SSPRC-1-2 and SSPRC-1-3 were confirmed as C15H1005 and C25H37NO4,the same as the known compound genistein and piericidin,respectively.Conclusion The MIC of two compounds SSPRC-1-1 and SSPRC-1-2 against P.oryzae were 125 and 24μg/mL.Compound SSPRC-1-3 showed good bioactive against Mythimna separate.