文献类型：期刊文献

英文题名：Real-Time Monitoring Renal Impairment Due to Drug-Induced AKI and Diabetes-Caused CKD Using an NAG-Activatable NIR-II Nanoprobe

作者：Tan, Jiahui[1];Yin, Kai[1];Ouyang, Zhirong[1];Wang, Rongchen[2,3];Pan, Hongming[1];Wang, Zhijun[1];Zhao, Chuchang[2,3];Guo, Wei[1];Gu, Xianfeng[1]

机构：[1]Fudan Univ, Sch Pharm, Dept Med Chem, Shanghai 201203, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：93

期号：48

起止页码：16158

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20214911272267);WOS:【SCI-EXPANDED(收录号:WOS:000753022600043)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21572039, 21977018, and 21874043) and the Shanghai Municipal Science and Technology Major Project (Grant No. 2018SHZDZX03).

语种：英文

外文关键词：Optical image storage - Nanoprobes - Fluorescence - Diagnosis - Infrared devices - Diseases

摘要：Real-time in vivo optical imaging of kidney function is important for the diagnosis of renal diseases, such as acute kidney injury (AKI) and chronic kidney disease (CKD), with high morbidity and mortality worldwide. However, the reported optical imaging agents still have limitations for identifying AKI or CKD in the early stage due to their low sensitivity, poor tissue penetration, and significant background interference. Herein, an N-acetyl-beta-D-glucosaminidase (NAG)-activatable second near-infrared (NIR-II) fluorescent nanoprobe (BOD-II-NAG-NP) is developed for monitoring the progression of drug-induced AKI and in vivo imaging of diabetes-caused CKD. NAG, as a biomarker of renal diseases, is able to specifically activate BOD-II-NAG-NP to release NIR-II fluorescence signals, enabling in vivo imaging of kidney dysfunctions in living mice. Importantly, such an active imaging mechanism allows BOD-II-NAG-NP to noninvasively detect the onset of drug-induced AKI at least 32 h earlier than the most existing assays, which indicates that BOD-II-NAG-NP has the potential to be an optical imaging agent for the early diagnosis of AKI. Moreover, NIR-II fluorescence produced by BOD-II-NAG-NP could deeply penetrate into the relatively thick layers of fat in diabetic nephropathy mice and provide in vivo imaging with high resolution, indicating that BOD-II-NAG-NP has clinical potential for precision diagnosis of CKD.