文献类型：期刊文献

英文题名：Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion

作者：Xu, Ke;Liang, Xin;Gao, Feng;Zhong, Jianjiang[1];Liu, Jianwen

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：45

期号：8

起止页码：1261

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000280382800007)】；

基金：This work was supported by Shanghai Leading Academic Discipline Project, Project No. B507, the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204, the modernization of Chinese medicine special project for Shanghai "Technology Innovation Action Plan", Grant No. 08DZ1971900 and the 111 Project, Grant No. B07023, and Shanghai Science and Technology Commission (No. 054319933) is gratefully acknowledged.

语种：英文

外文关键词：Ganoderic acid T; Metastasis; Invasion; MMPs; NF-kappa B

摘要：Ganoderma lucidum (GL) is known as a valuable herb in the search for anticancer lead compounds because it has been used for thousands of years as a traditional medication. Triterpenoids are the principal active components in this fungus. To examine the bioactivity of triterpene compounds, Ganoderic acid T (GA-T) was isolated from G. lucidum by high performance liquid chromatography (HPLC). In this study, we investigated the anti-invasion and antimetastasis mechanisms of GA-Tin vitro. GA-T dose-dependently inhibited 95-D cells migration by wound healing assay. Results of cell aggregation and adhesion assay showed that GA-T could promote cell aggregation and simultaneously inhibit cell from adhering to extracellular matrix (ECM). In addition, GA-T suppressed MMP-2 and MMP-9 gene expression, hence, decreased protein level of MMP-2 and MMP-9 in 95-D cells, according to RT-PCR assay and Western blot assay. These results demonstrate that GA-T effectively inhibits tumor metastasis in vitro. According to the result of Western blot, the suppression of NF-kappa B activation likely abrogates the expression of MMP-2 and MMP-9, thus prevents tumor metastasis. These results suggest that GA-T has therapeutic potential against high metastatic lung carcinoma as a new agent. (C) 2010 Elsevier Ltd. All rights reserved.