文献类型：期刊文献

英文题名：Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst

作者：Yu, Hui-Lei[1];Xu, Jian-He[1];Lu, Wen-Ya[2];Lin, Guo-Qiang[2]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Bioproc, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China

年份：2008

卷号：133

期号：4

起止页码：469

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20080611085862);WOS:【SCI-EXPANDED(收录号:WOS:000253875500008)】；

语种：英文

外文关键词：biocatalysis; apple seed meal; glycoside synthesis; salidroside; green chemistry

摘要：Salidroside is a natural glycoside with pharmacological activities of resisting anoxia, microwave radiation and fatigue, improving oxygen lack, and postponing ageing. In this work, salidroside and other natural glucosides such as cinnamyl O-beta-D-glucopyranoside and 4-methoxybenzyl O-beta-D-glucopyranoside were efficiently synthesized via an environmentally benign and energy economic process. In the synthetic process, apple seed, easily available from discards of fruit processing factories, was employed as a natural and green catalyst. Moreover, all of the catalyst, solvent and excessive substrate was reused or recycled. The biocatalytic reaction was carried out in a clean and less toxic medium of aqueous tert-butanol and the glucoside produced was selectively removed from reaction mixture by alumina column adsorption, making excessive substrate (aglycon) recyclable for a repeated use in the next batch of reaction. For improvement of the biocatalyst stability, apple seed meal was further cross-linked by glutaraldehyde, yielding a net-like porous structure within which the dissociating proteins were immobilized, resulting in improved permeability of the biocatalyst. After the simple cross-linking treatment, the half-life of apple seed catalyst was significantly improved from 29 days to 51 days. The productivity of the bioreactor in the case of salidroside can reach ca. 1.9 g l(-1) d(-1), affording the product in up to 99.3% purity after refinement. (C) 2007 Elsevier B.V. All rights reserved.