文献类型：期刊文献

英文题名：Cellulose nanocrystalline and sodium benzenesulfonate-doped polypyrrole nano-hydrogel/Au composites for ultrasensitive detection of carcinoembryonic antigen

作者：Huang, Xiangrong[1];Ni, Zhuoyao[1];Su, Haiping[1];Shang, Yazhuo[1];Liu, Honglai[1];He, Yifan[2];Meng, Hong[2];Dong, Yinmao[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Beijing Technol & Business Univ, Sch Sci, China Natl Light Ind, Key Lab Cosmet, Beijing 100048, Peoples R China

年份：2021

卷号：45

期号：12

起止页码：5551

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;EI(收录号：20211410174925);WOS:【SCI-EXPANDED(收录号:WOS:000634523300026)】；

基金：This work was supported by the Open Research Fund Program of Key Laboratory of Cosmetic (Beijing Technology and Business University), China National Light Industry (project no. KLC-2018-ZD1) and the National Natural Science Foundation of China for Innovative Research Groups (no. 51621002).

语种：英文

外文关键词：Nanocrystals - Cellulose - Gelation - Gold nanoparticles - Cellulose derivatives - Hydrogels - Infrared devices - Sodium - Glass membrane electrodes - Mammals - Immunosensors - Signal to noise ratio - Tumors

摘要：Precise and early detection of carcinoembryonic antigen (CEA) as a tumor marker of various cancers provides the best hope for the treatment of the deadly malignancy. Thus, the design of an immunosensor with high sensitivity is significant for early and accurate detection of CEA. In this work, a novel label-free electrochemical immunosensor was designed for the detection of CEA based on a nanostructured conductive polymer hydrogel loaded with gold nanoparticles (AuNPs). The hydrogel (BSNa-CNC-PPy) was simply prepared via in situ synthesis of polypyrrole (PPy) using cellulose nanocrystalline (CNC) and sodium benzenesulfonate (BSNa) as dopants to participate in the formation of the three-dimensional nano-hydrogel. AuNPs were further electrodeposited on the surface of BSNa-CNC-PPy gel-modified glassy carbon electrode (GCE). The immunoassay platform was obtained when the AuNPs/BSNa-CNC-PPy gel/GCE was incubated with anti-CEA and blocked by bovine serum albumin in succession. By combining the advantages of biocompatibility, nanostructure, outstanding hydrophilicity, excellent film-forming ability as well as the gelatinization of CNC, the CNC-PPy gel/GCE showed high conductivity (electroactive surface area), strong hydrophilicity, and film-forming property. The introduction of BSNa improved the conductivity of BSNa-CNC-PPy gel/GCE further due to enhanced interchain charge transport of PPy chains. The BSNa-CNC-PPy gel/GCE showed a 214% increase in electroactive surface area and the dried BSNa-CNC-PPy gel demonstrated a large specific surface area (76.59 m(2) g(-1)), which were superior to those of existing conducting polymer hydrogels. AuNPs were apt to immobilize a mass of antibodies and accelerate electron transfer. The fabricated CEA-detecting immunosensor displayed a wide linear detection range of 1 fg mL(-1) to 200 ng mL(-1) and an ultralow limit of detection of 0.06 fg mL(-1) (at a signal to noise ratio of 3). In addition to high sensitivity, the immunosensor exhibited excellent stability, high selectivity, good reproducibility, and strong reliability for real sample analysis. The proposed label-free electrochemical immunosensor has potential to be a diagnostic tool in clinical analysis of CEA.