文献类型：期刊文献

中文题名：基于多尺度参数相关分析的细胞培养过程优化与放大

英文题名：Cell culture process optimization and scale-up based on multi-scale parameter related analysis

作者：庄英萍[1];田锡炜[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2018

期号：1

起止页码：49

中文期刊名：生物产业技术

外文期刊名：Biotechnology & Business

基金：国家"863"计划项目(SS2015AA021005);上海市科委重点项目(15DZ2290500)

语种：中文

中文关键词：多尺度;参数相关分析;微观与宏观代谢;生理与流场特性;优化与放大

外文关键词：multi-scale; parameter related analysis; microscopic and macroscopic metabolism; physiological and flow field characteristics; optimization and scale-up

摘要：细胞大规模培养过程是活体细胞代谢的过程,因此存在着基因、细胞、反应器多尺度相关关系。通过对生物反应过程中生理代谢特性参数检测,并分析参数的理化相关和生物相关,可以实现多尺度观察与调控。主要介绍了在生物过程优化与放大研究中,整合分析与细胞生理代谢特性相关的参数变化信息以及反应器流场特性参数变化信息,从而实现微观与宏观相结合的过程优化方法以及细胞生理和反应器流场特性相结合的过程放大策略。
Due to the metabolism of living cells, multi-scale relationships exist among genetic, cellular and reactor levels in large scale cell culture process. Through detecting physiological and metabolic parameters, and subsequently analyzing their physical and chemical as well as biological relationships, multi-scale observation and regulation could be accomplished in bio-reaction process. This paper introduced that the integrated parameters characterizing cell physiological and metabolic characteristics as well as reactor flow field characteristics should be analyzed integrally for realizing bioprocess optimization and scale-up by the method of combining microscopic and macroscopic metabolism analysis and for achieving bioprocess scale-up by the method of combining cell physiological and fl ow fi eld characteristic analysis.