文献类型：期刊文献

英文题名：Exploring microbial degradation of polyamide 4 in soils: Unveiling degradation mechanisms, pathways, and the contribution of strain NR4

作者：Wang, Li[1,2,3];Zhang, Ziwei[1,2];Zhang, Di[1,2];Qiu, Yongjun[1,2,3];Wang, Yibing[1,2,3];Quan, Shu[1,4];Zhao, Liming[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Biobased Mat Engn, China Natl Light Ind, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai 200237, Peoples R China

年份：2023

卷号：429

外文期刊名：JOURNAL OF CLEANER PRODUCTION

收录：;EI(收录号：20234515016752);WOS:【SCI-EXPANDED(收录号:WOS:001116485700001)】；

基金：This work was supported by the Research and Development of Technical Standards for Bio-based Material Polybutyrolactam Project (21DZ2205900) , the National Key R & D Program of China (grant number 2022YFC2104500) , the 111 Project (B18022) , and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, ECUST (ZDXM2019) .

语种：英文

外文关键词：Polyamide 4; Biodegradation; Soil; Enrichment and acclimation; PA4 degrading mechanism

摘要：Evidence for microbial degradation of polyamide 4 (PA4) has previously been reported, but little is available on the specific strains and enzymes. The mechanism responsible for the degradation is still unknown. This study investigated the degradation behaviors of PA4 films in composted, garbage and campus soil. Various charac-terization methods were used to evaluate the changes in morphology, structure, crystallinity, and thermal properties of PA4 films during the soil biodegradation, to assess its degradation efficiency in four types of soils. The results showed that the highest degradation behavior of soil in composted soil that almost no residual films can be observed in this type of soil after 60 days, compared to those in garbage and campus soil. PA4 degradation resulting occurring in soils can enhance the population of certain beneficial microorganisms in the soil envi-ronment, such as Ensifer, Luteimonas, etc., which play a key role in nitrogen fixation, promoting plant growth, and carbon cycling. Moreover, a highly efficient single bacterium for PA4 degradation was isolated from the soil enriched and acclimated by PA4 films, named strain NR4. The complete genome of strain NR4 was sequenced to identify relevant enzyme-coding genes and metabolic pathways associated with PA4 degradation, ultimately leading to the proposal of a mechanism for PA4 biodegradation. The research not only examined how PA4 degrades in different types of soil but also suggested practical recommendations for promoting biodegradation of other products in the nylon family. The results obtained from the current study are essential in addressing environmental issues surrounding the buildup of non-biodegradable nylon items in the ecosystem.