文献类型：期刊文献

中文题名：蝉棒束孢菌孢梗束培植过程中主要成分含量的变化规律及其复方对小鼠睡眠的影响

英文题名：The content of main chemical constituents of coremium in different cultivation period of Isaria cicadae and the effects of compound prescription containing I. cicadae coremium on improving sleep of mice

作者：覃丽霞[1,2,3];王玉芹[4,5];李春如[4,5];王静春[4,5];孙长胜[4,5];王春丽[1,2,3]

机构：[1]华东理工大学药学院,上海200237;[2]制药工程与过程化学教育部工程研究中心,上海200237;[3]上海市新药设计重点实验室,上海200237;[4]浙江泛亚生物医药股份有限公司,浙江嘉兴314200;[5]浙江泛亚生命科学研究院,浙江嘉兴314200

年份：2020

卷号：39

期号：2

起止页码：362

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

语种：中文

中文关键词：蝉棒束孢菌孢梗束;多糖;腺苷;虫草酸;辅助睡眠实验

外文关键词：Isaria cicadae coremium;polysaccharides;adenosine;cordycepic acid;assisted sleep experiment

摘要：分析检测蝉棒束孢菌株BAI0101在培植过程中不同时间点孢梗束的转化率和孢梗束中多糖、虫草酸、腺苷和N6-(2-羟乙基)腺苷含量的变化规律,对配伍的孢梗束复方辅助改善睡眠功能进行了初步评价。结果表明,从培植18d起,孢梗束的转化率呈现上升趋势,在培植30d左右,有保持稳定趋势;孢梗束中的多糖、虫草酸和腺苷含量均随着培植时间的延长呈现增高趋势,均在22–24d达到最高值,其后开始呈现降低趋势,而孢梗束中的N6-(2-羟乙基)腺苷含量随着培植时间的延长呈现降低趋势,在24–26d左右达到最低值,其后呈现上升趋势,在培植至36d左右达到含量的最高值并保持稳定;对利用该菌株培植的孢梗束,经中药配伍组成的保健品小复方进行了辅助改善睡眠保健功能的初步评价,结果表明该复方有明显促进阈下剂量戊巴比妥钠诱导的小鼠睡眠作用,能明显降低巴比妥钠诱导小鼠睡眠潜伏期,且无直接睡眠作用,不影响戊巴比妥致小鼠睡眠时间,根据《保健食品检验与评价技术规范》,判定该复方具有辅助改善睡眠的保健功能。
Formation rate and the content of polysaccharides,cordycepin acid,adenosine and N6-(2-hydroxyethyl)adenosine of coremium of Isaria cicadae strain BAI0101 were detected and analyzed at different period during artificial cultivation.The effect of I.cicadae coremium compound on improving sleep of mice were evaluated preliminarily.The results showed that the coremium formation rate increased in 10–30 days of cultivation and then maintained a stable trend.The content of polysaccharides,cordycepin acid and adenosine began to increase in 18 days and reached the highest in 22–24 days,and then decreased.The content of N6-(2-hydroxyethyl)adenosine decreased with the prolongation of culture time,reached the lowest in 24–26 days,and then increased and kept stable in 36 days.Preliminary evaluation was made on the effect of the compound prescription consisting of I.cicadae coremium on improving sleeping condition of mice.The results showed that the compound could obviously promote the sleep of mice induced by pentobarbital sodium at subthreshold dose,and significantly reduce the sleep latency of mice induced by barbital sodium without direct sleep effect,and did not affect the sleep time of mice induced by pentobarbital.According to the‘Technical Specification for Inspection and Evaluation of Health Food’,the compound could be used as prescription for improving sleep.