文献类型：期刊文献

英文题名：Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

作者：Zhang, Min[1];Yin, Bin-Cheng[1];Wang, Xian-Fei[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2011

卷号：47

期号：8

起止页码：2399

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287091100056)】；

基金：This work was financially supported by NSF21075040, the Shanghai 09JC1404100, NCET-07-0287, SKLBE Fund 2060204, and the Fundamental Research Funds for the Central Universities. We are grateful to Prof. Huang-Hao Yang for kindly providing graphene oxide.

语种：英文

摘要：We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.