文献类型：期刊文献

英文题名：Rapid bio-barcode assay for multiplex DNA detection based on capillary DNA Analyzer

作者：He, MingXing[1,2];Li, Kai[1];Mao, JunHua[1];Zhou, YuXun[1,2]

机构：[1]DongHua Univ, Inst Biol Sci & Technol, Shanghai 201620, Peoples R China;[2]E China Univ Sci & Technol, Inst Bioengn, Coll Biochem & Mol Biol, Shanghai 200237, Peoples R China

年份：2008

卷号：151

期号：1

起止页码：126

外文期刊名：JOURNAL OF VIROLOGICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257639700020)】；

语种：英文

外文关键词：DNA detection; nanogold particles; bio-barcode assay

摘要：The detection of virus at low copy number is important for clinical diagnosis. In this study, a rapid bio-barcode assay was developed and it could detect short sequences of four types of virus DNA simultaneously at the concentration as low as 5 pmol/L in 40 min with capillary 3730 DNA Analyzer. The background of the assay using high salt concentration prepared nanogold particle probes was five times less than that of the assay using conventionally prepared probes. With further optimization, the specificity of the complementary strands to the noncomplementary strands observed in the assay approached 140:1. Compared with the conventional bio-barcode assay, the current assay provides an alternative enzyme and labor-free selection of timesaving, better sensitivity and specificity, as well as higher throughput. (C) 2008 Elsevier B.V. All rights reserved.