文献类型：期刊文献

英文题名：High-Fidelity Trapping of Spatial-Temporal Mitochondria with Rational Design of Aggregation-Induced Emission Probes

作者：Zhang, Jie[1];Wang, Qi[1];Guo, Zhiqian[1];Zhang, Shaoze[1];Yan, Chenxu[1];Tian, He[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Sch Chem & Mol Engn,Key Lab Adv Mat & Inst Fine C, Feringa Nobel Prize Scientist Joint Res Ctr,Shang, Shanghai 200237, Peoples R China

年份：2019

卷号：29

期号：16

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20191706816832);WOS:【SCI-EXPANDED(收录号:WOS:000467385200009)】；

基金：J.Z. and Q.W. contributed equally to this work. This work was supported by NSFC Science Center Program (21788102) and Creative Research Groups (21421004), National key Research and Development Program (2016YFA0200300 and 2017YFC0906900), NSFC/China (21636002, 21622602 and 81602718), National Postdoctoral Program for Innovative Talents (BX201700075), Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), Fundamental Research Funds for the Central Universities, and Program of Introducing Talents of Discipline to Universities (B16017).

语种：英文

外文关键词：aggregation-induced emission; high-fidelity trapping; image-guided therapy; mitochondrial imaging; "off-on" fluorescence

摘要：High-fidelity trapping of mitochondrial dynamic activity is critical to value cellular functions and forecast disease but lack of spatial-temporal probes. Given that commercial mitochondria probes suffering from low photostability, aggregation-caused quenching effect, and limited signal-to-noise ratio from fluorescence always on in the process of targeting mitochondria, here, the rational design strategy of a novel aggregation-induced emission (AIE) molecular motif and unique insight into the high-fidelity targeting of mitochondria is reported, thereby illustrating the relationship between tailoring molecular aggregation state and mitochondrial targeting ability. This study focuses on how to exactly modulate the hydrophilicity and the aggregated state for realizing "off-on" fluorescence, as well as matching the charge density to go across the cell membrane for mitochondrial targeting. Probe tricyano-methylene-pyridine (TCM-1) exhibits an unprecedented high-fidelity feedback on spatial-temporal mitochondrial information with several advantages such as "off-on" near-infrared characteristic, high targeting capacity, favorable biocompatibility, as well as excellent photostability. TCM-1 also produces reactive oxygen species in situ for image-guided photodynamic anticancer therapy. Through unraveling the relationship between tuning molecular aggregation behavior and organelle-specific targeting ability, for the first time, a unique guide is provided in designing AIE-active probes to explore the hydrophilicity and membrane potential for targeting subcellular organelles.