文献类型：期刊文献

中文题名：不动杆菌生物合成新型羧酸酯酶的摇瓶培养条件优化

英文题名：Optimization of Acinetobater sp. Culture Conditions in Shake Flasks for Efficient Biosynthesis of a Novel Carboxylesterase

作者：赵玉巧[1];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2005

卷号：19

期号：4

起止页码：523

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;EI(收录号：2005369347027);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家重点基础研究发展(973)计划项目(2003CB716008);教育部优秀青年教师培养计划资助

语种：中文

中文关键词：(R)-烯丙醇酮乙酸酯;羧酸酯酶;不动杆菌;产酶条件

外文关键词：Biosynthesis;Carboxylation;Cultivation;Fermentation;Hydrolysis;Optimization;Production

摘要：对一株产(R)-烯丙醇酮乙酸酯对映选择性水解酶的不动杆菌(Acinetobactersp.CGMCC0789)摇瓶发酵产酶条件进行了优化。培养基最佳组成为(g?L?1):蔗糖8.5,吐温-801.5,黄豆饼粉20.0,(NH4)2SO42.0,K2HPO42.0,NaCl1.0,MgSO4·7H2O0.2;优化后酯酶产量提高了8.3倍(由46增至427U?L?1)。添加吐温-80可以诱导酶的合成。摇瓶培养最佳条件为:装液量15%,发酵起始pH8.0,接种量6%,发酵温度30℃。考察了细胞生长及产酶的时间进程,最佳培养时间为30h.
The culture conditions of Acinetobacter sp. strain CGMCC 0789 were optimized for the production of a Carboxylesterase which is useful for the enantioselective hydrolysis of racemic 4-hydroxy-3-methyl-2-(2-propenyl)-cyclopent-2-enone (rac-HMPC) acetate. The optimum medium (per liter) consistes of 8.5 g sucrose, 1.5 g Tween-80, 20 g soy bean meal, 2.0 g (NH4)2SO4, 2.0 g K2HPO4, 1.0 g NaCl, and 0.2 g MgSO4 · 7H2O. After optimization of the culture medium, the esterase production in shake flasks is enhanced from 46 to 427 U · L-1. The esterase production is increased significantly when a small amount (1.5 g · L-1) of Tween-80 was added. The optimum fermentation conditions were determined as follows: inoculum size 6%, temperature 30°C, medium volume 37.5 mL (i.e., 15% for a 250-mL shake flask), initial pH 8.0, and cultivation time 30 h.