文献类型：期刊文献

英文题名：In vitro and animal study of novel nano-hydroxyapatite/poly(Ε -caprolactone) composite scaffolds fabricated by layer manufacturing process

作者：Heo, Su-Jin[1,2]; Kim, Seung-Eon[2]; Wei, Jie[3]; Kim, Dong Hwa[1]; Hyun, Young-Taek[2]; Yun, Hui-Suk[2]; Kim, Hyung Keun[4]; Yoon, Taek Rim[4]; Kim, Su-Hyang[1]; Park, Su-A[5]; Shin, Ji Won[1]; Shin, Jung-Woog[1,6,7]

机构：[1] Team of BK21, Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam, Korea, Republic of; [2] Future Technology Center, Korea Institute of Materials Science, Changwon, Gyeongnam, Korea, Republic of; [3] Institute of Biomaterials, East China University of Science and Technology, Shanghai, China; [4] Department Orthopedics, Chonnam National University Hwasun Hospital, Ilsimri Hwasun-Hwasun-gun Jeonnam, Korea, Republic of; [5] Nano-Mechanical Systems Research Division, Korea Institute of Machinery and Materials, Jang-Dong, Daejeon, Korea, Republic of; [6] FIRST Research Group, Institute of Biomedical Engineering, Inje University, Gimhae, Gyeongnam, Korea, Republic of; [7] Team of BK21, Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Korea, Republic of

年份：2009

卷号：15

期号：5

起止页码：977

外文期刊名：Tissue Engineering - Part A

收录：EI(收录号：20092412119387)

语种：英文

外文关键词：Cell culture - Pore size - Biocompatibility - Nanocomposites - Phosphatases - Scaffolds (biology) - Stem cells - Bone - Hydroxyapatite - Animals - Particle size

摘要：The purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventional scaffolds made by salt leaching and gas forming process was investigated through animal study. Using the LMP, we have created a new nano-sized hydroxyapatite/ poly(Ε-caprolactone) composite (n-HPC) scaffold and a micro-sized hydroxyapatite/poly(Ε-caprolactone) composite (m-HPC) scaffold for bone tissue engineering applications. The scaffold macropores were well interconnected, with a porosity of 73% and a pore size of 500 μm. The compressive modulus of the n-HPC and m-HPC scaffolds was 6.76 and 3.18 MPa, respectively. We compared the cellular responses to the two kinds of scaffolds. Both n-HPC and m-HPC exhibited good in vitro biocompatibility. Attachment and proliferation of mesenchymal stem cells were better on the n-HPC than on the m-HPC scaffold. Moreover, significantly higher alkaline phosphatase activity and calcium content were observed on the n-HPC than on the m-HPC scaffold. In an animal study, the LMP scaffolds enhanced bone formation, owing to their well-interconnected pores. Radiological and histological examinations confirmed that the new bony tissue had grown easily into the entire n-HPC scaffold fabricated by LMP. We suggest that the well-interconnected pores in the LMP scaffolds might encourage cell attachment, proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the LMP scaffolds. This study shows that bioactive and biocompatible n-HPC composite scaffolds prepared using an LMP have potential applications in bone tissue engineering. ? 2009, Mary Ann Liebert, Inc.