文献类型：期刊文献

英文题名：Preparation of (S)-mandelic acid from racemate using growing cells of Pseudomonas putida ECU1009 with (R)-mandelate degradation activity

作者：Huang, Han-Rong[1]; Xu, Jian-He[1]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2006

卷号：30

期号：1

起止页码：11

外文期刊名：Biochemical Engineering Journal

收录：EI(收录号：2006189859800)

语种：英文

外文关键词：Batch cell culture - Biodegradation - Cells - Optimization - Yeast

摘要：(S)-Mandelic acid [(S)-MA] was prepared in a one-pot fermentation-transformation process by a (R)-MA-degrading bacterium, Pseudomonas putida ECU1009. Using a sequential simplex optimization method, the best results were obtained with growing cells cultivated on a medium composed of 1.0% (RS)-MA, 0.1% NH4NO3, and 0.1% corn steep liquor. The optimal cultivation conditions were 30 °C and pH 6.5. By fed-batch culture under the optimal conditions, (S)-MA was successfully prepared in 1-L Erlenmeyer flask with 41% isolation yield and >99.9% ee, from totally 3% (RS)-MA after 94 h of biotransformation. ? 2006 Elsevier B.V. All rights reserved.