文献类型：期刊文献

中文题名：Co^(2+)对红霉素重组工程菌ZL1004发酵过程的影响

英文题名：The effect of Co^(2+) on the fermentation process of recombinant industrial strain Saccharopolyspora erythraea ZL1004

作者：尚永崇[1];钱江潮[1];黄明志[1];储炬[1];庄英萍[1];张嗣良[1];李万钧[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]宜都东阳光生化制药有限公司,宜昌443300

年份：2010

卷号：35

期号：12

起止页码：898

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家高新技术研究计划(863重点项目;2006AA020304);国家科技支撑项目(2007BAI26B02;2008BAI63B01)

语种：中文

中文关键词：红霉素;Co2+;重组工程菌;组分

外文关键词：Erythromycin; Co^2＋; Recombinant strain; Components

摘要：在50L发酵罐上考察了重组工程菌ZL1004的组分情况,与出发菌相比,A组分由75.43%上升到87.85%,B组分由4.42%下降到0.45%,C组分由13.35%下降到1.70%,而首次关注的红霉素E和F含量,却分别升高了47.0%和48.6%。表明改造后的工程菌,随着A组分的大量提升,部分红霉素A进一步转化形成了红霉素E和F等杂质组分。通过在0h添加0.04g/L CoCl2.6H2O,重组菌的红霉素合成代谢流明显加强。发酵结束时,与对照相比,红霉素效价提高12.4%,其中A组分含量由7766U/mL升高至9177U/mL,杂质组分红霉素E和F的百分比分别下降了23.8%和15.6%。研究结果表明Co2+能有效提高重组工程菌ZL1004的A组分含量,同时抑制红霉素A进一步转化为红霉素E和F。
The erythromycin components of recombinant strain Saccharopolyspora erythraea ZL1004 fermentation were investigated in 50L fermenter.In comparison with the wild type strain,the ErA was improved from 75.43% to 87.85%,the ratio of ErB was decreased significantly from 4.42% to 0.45%,the ratio of ErC was decreased greatly from 75.43% to 87.85%,but the ErE and ErF,which are the other two impurities of erythromycin,were increased by 47.0% and 48.6% respectively.These results indicated that some amount of ErA was further slowly transformed to ErE and ErF during the process of ErA biosynthesis.The metabolic flux of erythromycin biosynthesis was enhanced obviously by adding 0.04g/L CoCl2·6H2O.In comparison with the control.The maximal ErA production was increased from 7766 U/mL to 9177 U/mL at 186h,while the ratio of ErE and ErF were decreased by 23.8% and 15.6% respectively.The results showed that Co^2＋ can increase the production of ErA effectively and suppress ErA further convert to ErE and ErF.