文献类型：期刊文献

英文题名：Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells

作者：Jiang, Lei[1,2];Chen, Hua-Ying[1];He, Cai-Hong[1];Xu, Han-Bin[1];Zhou, Ze-Rui[1];Wu, Man-Sha[1];Fodjo, Essy Kouadio[1,3];He, Yue[1];Hafez, Mahmoud Elsayed[1,4];Qian, Ruo-Can[1];Li, Da-Wei[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Hangzhou Normal Univ, Coll Mat Chem & Chem Engn, Key Lab Organosilicon Chem & Mat Technol, Minist Educ, Hangzhou 311121, Zhejiang, Peoples R China;[3]Felix Houphouet Boigny Univ, Lab Phys Chem, Abidjan 225, Cote Ivoire;[4]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2023

卷号：95

期号：6

起止页码：3507

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20230613560502);WOS:【SCI-EXPANDED(收录号:WOS:000927525700001)】；

基金：ACKNOWLEDGMENTS This work was financially supported by the National Natural Science Foundation of China (grant nos 21974046 and 22176058) , Science and Technology Commission of Shanghai Municipality (grant nos 19391901700 and 19520744000) , and the Fundamental Research Funds for the Central Universities. The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the characterization of SEM images.

语种：英文

外文关键词：Cancer cells - Metabolism - Nanoprobes - Nanorods - Organometallics - Oxygen - Raman spectroscopy - Visualization

摘要：: ATP and reactive oxygen species (ROS) are considered significant indicators of cell apoptosis. However, visualizing the interplay between apoptosis-related ATP and ROS is challenging. Herein, we developed a metal-organic framework (MOF)-based nanoprobe for an apoptosis assay using duplex imaging of cellular ATP and ROS. The nanoprobe was fabricated through controlled encapsulation of gold nanorods with a thin zirconium-based MOF layer, followed by modification of the ROSresponsive molecules 2-mercaptohydroquinone and 6-carboxyfluorescein-labeled ATP aptamer. The nanoprobe enables ATP and ROS visualization via fluorescence and surface-enhanced Raman spectroscopy, respectively, avoiding the mutual interference that often occurs in single-mode methods. Moreover, the dual-modal assay effectively showed dynamic imaging of ATP and ROS in cancer cells treated with various drugs, revealing their apoptosis-related pathways and interactions that differ from those under normal conditions. This study provides a method for studying the relationship between energy metabolism and redox homeostasis in cell apoptosis processes.