文献类型：期刊文献

英文题名：Establishment of a search library about benzylisoquinoline alkaloids based on selective separation on the binaphthyl column and standard analysis on C18 column

作者：Liu, Qiaoxia[1];Zhou, Binbin[1];Wang, Xinliang[1];Ke, Yanxiong[1];Jin, Yu[1];Yin, Lihui[2];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China;[2]NIFDC, Beijing, Peoples R China

年份：2012

卷号：35

期号：23

起止页码：3317

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20124915747116);WOS:【SCI-EXPANDED(收录号:WOS:000311574500009)】；

基金：This work was supported by National Natural Science Funds for Distinguished Young Scholars (20825518) and National Natural Science Funds (21005028).

语种：英文

外文关键词：Benzylisoquinoline alkaloids; Binaphthyl column; C18 column; Search library; Selective separation

摘要：A search library about benzylisoquinoline alkaloids was established based on preparation of alkaloid fractions from Rhizoma coptidis, Cortex phellodendri, and Rhizoma corydalis. In this work, two alkaloid fractions from each herbal medicine were first prepared based on selective separation on the click binaphthyl column. And then these alkaloid fractions were analyzed on C18 column by liquid chromatography coupled with tandem mass spectrometry. Many structure-related compounds were included in these alkaloids fractions, which led to easy separation and good MS response in further work. Therefore, a search library of 52 benzylisoquinoline alkaloids was established, which included eight aporphine, 19 tetrahydroprotoberberine, two protopine, two benzyltetrahydroisoquinoline, and 21 protoberberine alkaloids. The information of the search library contained compound names, structures, retention times, accurate masses, fragmentation pathways of benzylisoquionline alkaloids, and their sources from three herbal medicines. Using such a library, the alkaloids, especially those trace and unknown components in some herbal medicine could be accurately and quickly identified. In addition, the distribution of benzylisoquinoline alkaloids in the herbal medicines could be also summarized by searching the source samples in the library.