文献类型：期刊文献

英文题名：Rapid determination of cysteine and chiral discrimination of D-/L-cysteine via the aggregation-induced emission enhancement of gold nanoclusters by Ag+

作者：Ruan, Shengli[1];Zhou, Yan[2];Zhang, Min[2];Zhang, Hongyang[1];Wang, Yuerong[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai Key Lab New Drug Design,Sch Pharm Dept, Shanghai 200237, Peoples R China

年份：2022

卷号：38

期号：3

起止页码：541

外文期刊名：ANALYTICAL SCIENCES

收录：;EI(收录号：20222612272916);WOS:【SCI-EXPANDED(收录号:WOS:000808551900009)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81973285 and 81573397) and Shanghai Natural Science Foundation (15ZR1409400).

语种：英文

外文关键词：Cysteine; Gold nanoclusters; Silver ion; Aggregation-induced emission enhancement; Chiral recognition

摘要：Cysteine (Cys) plays vital roles in various physiological and pathological functions. Either a deficiency or excess of Cys could lead to severe ailments in human. The identification and determination of Cys are the key issues for the early diagnosis of relevant diseases. This contribution has presented a promising potential of fluorescent gold nanoclusters (AuNCs) for Cys determination and D-/L-Cys enantiomer discrimination. Cys determination and discrimination are involved three steps. First, as a reducing and capping ligand, glutathione was applied to fabricate weak fluorescent AuNCs. Second, Ag+ was introduced to lead the aggregation-induced emission (AIE) to form well-dispersed aggregates. The fluorescence intensity of AuNCs was monitored at excitation/emission wavelengths of 396/620 nm. Third, Cys was found to quickly bind with Ag+ to form a grid network to light up the system via aggregation-induced emission enhancement (AIEE). A novel sensor for a sensitive and a visually selective detection of Cys was established on the basis of the AIEE mechanism. Rapid quantitative determination of Cys was achieved in 2 min via AIEE within the range of 0.5-100 mu mol L-1 and a detection limit of 0.365 mu mol L-1. Moreover, due to the specific interactions of D-/L-Cys with mandelic acid and tartaric acid, the visual discrimination of D-/L-Cys enantiomers with naked eyes was realized by replacing the organic acid buffer.