文献类型：期刊文献

中文题名：当归中阿魏酸的提取和纯化研究

英文题名：Study on the Extraction and Purification of Ferulic Acid in Angelica Sinensis

作者：刘芝兰[1];王娟[2];沈平[2];沈永嘉[1]

机构：[1]华东理工大学精细化工研究所结构可控先进功能材料及其制备教育部重点实验室,上海200237;[2]国家中药制药工程研究中心,上海201203

年份：2007

卷号：21

期号：6

起止页码：1008

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;EI(收录号：20080411058681);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：微波辅助提取;阿魏酸;当归;均匀设计;高速逆流色谱

外文关键词：microwave assisted extraction; ferulic acid; Angelica sinensis; uniform design; high-speed counter-current chromatography

摘要：以当归中主要活性物质阿魏酸的含量为指标,采用单因素及均匀设计实验的方法,确定了微波辅助提取当归中阿魏酸的最优工艺条件:乙醇浓度90%,微波功率850W,液固比6:1(mL:g),辐射时间9min,粒径大小为2mm,浸泡时间30min。结果表明:干浸膏中阿魏酸含量为0.212%,转移率为70.19%,均优于传统加热提取和超声提取方法,而且所用的时间分别是传统加热提取方法和超声提取方法的3/40和3/10。将高速逆流色谱技术应用于上述干浸膏中阿魏酸的分离纯化,经过一次分离得到的阿魏酸纯度为98.2%(HPLC)。HSCCC的溶剂系统组成为:正己烷-乙酸乙酯-甲醇-水(3:7:5:5)。用核磁共振和质谱表征了所得阿魏酸的化学结构。
The ferulic acid is one of the main active components in Angelica sinensis （Danggui）, and the microwave-assisted extraction was used for the extraction of ferulic acid from Danggui. The operation technological conditions of the microwave-assisted extraction were optimized by single factor experiments and uniform design method. The optimized technological parameters found are as follows： the 90% ethanol is used as solvent, the ratio of solvent to solid material is 6：1 （mL： g）, soak time is 30 min, the microwave irradiation time is 9 min and the microwave power is 850 W. Using the above optimum parameters, the extraction yield reaches 70.19% and the ferulic acid content in the dry powder obtained from the extraction of Danggui is 0.212%, these values are higher than that obtained by traditional heat-water （80℃） extraction and ultrasonic extraction method. The extraction time needed for microwave -assisted extraction is only 3/40 of that needed for heat-water extraction and is 3/10 of that needed for ultrasonic extraction method. Furthermore, using the high-speed counter-current chromatography （HSCCC） with the solvent system of n-hexane-ethyl acetate-methanol-water （3：7：5：5, v/v）, the dry powder obtained from the extraction of Danggui was purified and the ferulic acid with purity of 98.2% was obtained. The purity mentioned was assayed by HPLC and its chemical structure was confirmed by using Mass and 1↑H-NMR.