文献类型：期刊文献

英文题名：Charge-regulated hepatic γ-glutamyltranspeptidase fluorescent probe: in vivo staging of Schistosoma infection

作者：Ma, Xiaoxi[1,2];Xie, Eryang[1,2];Li, Qiang[4];Sun, Chuyang[1,2];Yao, Yongkang[1,2];Yan, Chenxu[1,2];Luo, Qianfu[1,2];Guo, Zhiqian[1,2,3];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Inst Fine Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Natl Hlth Commiss, Natl Inst Parasit Dis Chinese Ctr Dis Control & Pr, Natl Key Lab Intelligent Tracking & Forecasting In, Chinese Ctr Trop Dis Res,Natl Res Ctr Trop Dis, Shanghai 20025, Peoples R China

年份：2026

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20262020703399);WOS:【SCI-EXPANDED(收录号:WOS:001763015200001)】；

基金：This work was supported by the National Key Research and Development Program (2023YFA1802000), NSFC/China (22225805, 32121005, T2522013, 32394001, and 22378122), Shanghai Science and Technology Innovation Action Plan (No. 23J21901600), Innovation Program of the Shanghai Municipal Education Commission, Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, Grant 2021 Sci & Tech 0328), the Science and Technology Commission of Shanghai Municipality (24DX1400200), and the Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China (JYB2025XDXM404).

语种：英文

外文关键词：Diagnosis - Health risks - Liver - Probes - Screening

摘要：Schistosomiasis remains a formidable global health threat, yet with the limited sensitivity and critical inability of real-time in vivo monitoring using current diagnostic modalities such as microscopy and ultrasonography, there are significant hurdles to overcome before precise infection staging can be performed. To address this diagnostic bottleneck, we developed a de novo strategic charge-regulation approach with a dual-channel near-infrared fluorescent probe that binds to hepatic gamma-glutamyltranspeptidase (GGT), a key biomarker for schistosomiasis-induced liver pathological evolution. By engineering quinoline scaffolding from zwitterionic, single-positive charge, to double-positive charge, the optimized probe QMC-N-GGT achieved superior precise targeting of infected liver tissues in an anionic microenvironment. Impressively, its breakthrough dual-channel signals enable tracking of when, where, and how the probe targets the liver and illuminates endogenous GGT in situ. This probe exhibits a remarkable stage-dependent fluorescent response to GGT, enabling accurate distinction of slight, moderate, and severe infection stages with an ultra-high signal-to-noise ratio. QMC-N-GGT thus represents an unprecedented diagnostic tool, bridging the gap between conventional infection screening and advanced pathological staging for non-invasive, real-time schistosomiasis monitoring.