文献类型：期刊文献

中文题名：阿胶酶解成分对贫血小鼠造血系统的保护机制

英文题名：Fractions Prepared from Digested Colla Corri Asini and Its Hematopoietic Mechanism on the Anemic Mice

作者：吴宏忠[1];杨帆[1];崔书亚[1];秦玉峰[2];张元兴[1];刘建文[3]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]山东东阿阿胶集团,山东聊城252201;[3]华东理工大学药学院,上海200237

年份：2008

卷号：34

期号：1

起止页码：47

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20081311171210);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(20572022);山东东阿阿胶股份有限公司中药现代化项目

语种：中文

中文关键词：阿胶;活性成分制备;补血机理

外文关键词：colla corri asini; active fraction preparation; hematopoiesis mechanism

摘要：运用5-氟脲嘧啶制备的小鼠贫血模型研究降解分离后所得阿胶有效组分A、B的升高红、白血球的作用机制。结果显示:体外消化液中提取的A、B组分能促进贫血小鼠外周血白细胞和红细胞的升高,促进骨髓和脾造血干/祖细胞集落BFU-E,CFU-E,CFU-GM的增加,提高外周血GM-CSF,IL-6,EPO的含量,降低负相造血因子INF-γ、TGF-β含量,刺激肝和肾EPO和GM-CSF mRNA表达。从而说明从体外模拟人胃、肠的消化系统分离的阿胶组分A、B能够刺激造血。该有效补血活性成分的升高红、白血球的作用机制可能与保护贫血小鼠造血微环境和造血干、祖细胞,刺激髓外造血器官相关造血细胞因子表达有关。
The anemic mice model induced by 5-fluorouracil （5-Fu） was used to explore the possible hematopietic mechanism of fraction A and B from enzymed-digested colla corii asini on erythrocytes and granulocytes. Results show that fraction A and B can improve erythrocytes and granulocytes number in the peripheral blood, protect the hematopoeitc stem ceils colonies forming unit BFU-E, CFU-E and CFU-GM in the bone marrow and spleen, enhance the level of GM-CSF and IL-6 and EPO, decrease the level of INF-α and TGF-β in the serum and stimulate the mRNA expression of GM-CSF and EPO in the kidney and liver. These results suggest that simulated human gastric and intestinac digestion model is useful for the separation of active components from the protein related drugs. The possible hematopoietic mechanism of colla corii asini is associated with the protection of hematopoeitic microenviroment and activation of auxiliary hemapoietic organs, liver and spleen and kidney.