文献类型：期刊文献

中文题名：一种脂肽类生物表面活性剂产生菌的筛选

英文题名：Isolation and Screening of Lipopeptide Biosurfactant Producing Bacterium

作者：吕应年[1];杨世忠[1];牟伯中[1]

机构：[1]华东理工大学化学系

年份：2005

卷号：25

期号：2

起止页码：4

中文期刊名：微生物学杂志

外文期刊名：Journal of Microbiology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：国家自然科学基金(59974014);教育部博士点基金(20030251002);教育部重点项目(03071);上海市教委资助项目

语种：中文

中文关键词：枯草芽孢杆菌;筛选;生长特性;生物表面活性剂;脂肽

外文关键词：Bacillus subtilis; screening; characterization; biosurfactant; lipopeptide

摘要：从油田地层水中筛选分离得到1株能够产生表面活性剂的细菌,经鉴定为枯草芽孢杆菌。分析了该菌株的生理形态和生长特性,以及该菌株代谢产生的生物表面活性剂的性质。薄层色谱与原位水解显色和红外光谱分析表明,培养后菌株代谢产生的生物表面活性为脂肽。它能使水的表面张力降低到26mN/m,其临界胶束浓度为0.025mg/mL。
A biosurfactant producing bacterium was isolated and screened from aquifer stratum in an oil field and {identified} as Bacillus subtilis. Its physiological morphology, growth, and the biosurfactant properties of metabolite it produced were analyzed. Analysis by thin layer chromatography, original spot hydrolyzed-ninhydrin color, infrared spectrum indicated that the biosurfactant of metabolite after culturing of the strain was lipopeptide. It can reduce surface tension of water to 26 mN/m, and its critical micelle concentration (CMC) was 0.025 mg/mL.