文献类型：期刊文献

英文题名：The protective effect of ascorbic acid derivative on PC12 cells: Involvement of its ROS scavenging ability

作者：Du, CB; Liu, JW; Su, W; Ren, YH; Wei, DZ

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2003

卷号：74

期号：6

起止页码：771

外文期刊名：LIFE SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000187673600008)】；

语种：英文

外文关键词：L-ascorbic acid-2-phosphate-6-palmitate; antioxidant; reactive oxygen species; hydrogen peroxide; neurodegenerative disease

摘要：L-ascorbic acid 2-phosphate-6-palmitate (Asc2P6P) was synthesized and its effect on the damage of PC12 cells induced by H2O2 was investigated. 200 muM H2O2 in a treatment period of 4 hours in our experiment resulted in substantial cell loss. With the. increasing concentration of antioxidants, such H2O2-induced cytotoxicity was significantly prevented and the corresponding intracellular and extracellular ROS levels decreased concurrently by pre-treatment with Asc2P6P and Asc. It was found that Asc2P6P was superior to L-ascorbic acid in its protective role and showed a dose-dependent manner during a 24-hour treatment. The higher potency of Asc2P6P's protective role on PC12 cells was correlated with its more effective ROS scavenging ability. HPLC assay demonstrated that Asc2P6P could easily enter the cells and be converted into Asc persistently, which contributed to its distinguished role in protecting PC12 cells against H2O2-induced cytotoxicity. (C) 2003 Elsevier B.V All rights reserved.