文献类型：期刊文献

英文题名：The effect of surfactant on fermentation of kitasamycin in Streptomyces kitasatoensis

作者：Zheng, Qianli[1];Gao, Shuhong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：63

期号：6

起止页码：895

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;EI(收录号：20154701573256);WOS:【SCI-EXPANDED(收录号:WOS:000392814600017)】；

基金：This work was supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. The authors declare that they have no competing interests.

语种：英文

外文关键词：component; kitasamycin; production; SDS; Streptomyces kitasatoensis; surfactant

摘要：Soybean oil is an important carbon source in kitasamycin fermentation by Streptomyces kitasatoensis. In this study, three different surfactants, Tween 80, Tween 85, and sodium dodecyl sulfate (SDS), were added in the fermentation medium to improve soybean oil utilization. Results indicated that all of these surfactants promote kitasamycin biosynthesis. When 0.5 g/L SDS was added at the beginning of fermentation, kitasamycin production increased by 55% and A(5) content improved by 12%, compared with the control treatment (i.e., no surfactant added). Oil consumption rate and lipase activity were also improved in the presence of SDS, producing more organic acids benefiting kitasamycin biosynthesis. High butyric acid concentration in the fermentation medium containing SDS repressed C-3 acetylation and promoted A(5) component accumulation. Additionally, utilization of oil components by S. kitasatoensis was altered. Specifically, linoleic acid was primarily used in the fermentation process with SDS, whereas oleic acid was primarily used in the fermentation process where no surfactant had been added. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc. Volume 63, Number 6, Pages 895-900, 2016