文献类型：期刊文献

英文题名：Biosynthesis of aspergiolide A, a novel antitumor compound by a marine-derived fungus Aspergillus glaucus via the polyketide pathway

作者：Tao, Kejing[1];Du, Lin[2];Sun, Xueqian[1];Cai, Menghao[1];Zhu, Tianjiao[2];Zhou, Xiangshan[1];Gu, Qianqun[2];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ocean Univ China, Key Lab Marine Drugs, Chinese Minist Educ, Inst Marine Drugs & Food, Qingdao 266003, Peoples R China

年份：2009

卷号：50

期号：9

起止页码：1082

外文期刊名：TETRAHEDRON LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000263749400032)】；

基金：This work was financially supported by the National High Technology Research and Development Program of China (No. 2006AA09Z429).

语种：英文

外文关键词：Aspergillus glaucus; Biosynthesis; Polyketide pathway; C-13 NMR; Feeding experiments; Natural products

摘要：Aspergiolide A, a novel antitumor compound, was produced by a marine-derived filamentous fungus Aspergillus glaucus. The biosynthesis of it was unambiguously determined by feeding experiments using [1-C-13]sodium acetate, [2-C-13]sodium acetate, and [1,2-C-13(2)]sodium acetate precursors followed by C-13 NMR spectroscopic investigation of the isolated products. Analysis of the patterns of C-13-enrichment revealed that all 25 carbon atoms in skeleton of aspergiolide A were derived from labeled acetate. And among them, 12 carbon atoms were labeled from the carboxylic group of acetate, while the other 13 carbon atoms were labeled from the methylic group of acetate. Besides, the labeling pattern of [1,2-C-13(2)]sodium, acetate feeding experiment demonstrated that 12 intact acetate units were incorporated in aspergiolide A by polyketide pathway. (c) 2008 Elsevier Ltd. All Fights reserved.