文献类型：期刊文献

英文题名：Biochemical properties of a novel chitosanase from Bacillus amyloliquefaciens and its use in membrane reactor

作者：Qin, Zhen[1,2];Luo, Sa[1];Li, Yun[1];Chen, Qiming[1,2];Qiu, Yongjun[1,2];Zhao, Liming[1,2];Jiang, Lihua[1,2];Zhou, Jiachun[1,2]

机构：[1]East China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]SCICBT, Shanghai 200237, Peoples R China

年份：2018

卷号：97

起止页码：9

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20182605359779);WOS:【SCI-EXPANDED(收录号:WOS:000445715600002)】；

基金：This work was financially supported by the National Natural Science Foundation of China (31701537), China Postdoctoral Science Foundation (2016M601530), Shanghai Sailing Program (17YF1403500), Fundamental Research Funds for the Central Universities (222201814031) and "Shu Guang" project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (15SG28).

语种：英文

外文关键词：Chitosanase; Chitooligosaccharides; Glycoside hydrolase family 46; Enzyme membrane reactor; Product selectivity

摘要：Chitooligosaccharides are widely used in functional food, medicaments and agriculture. Controllable conversion of chitosan to chitooligosaccharides with desired degree of polymerization (DP) is of great value. Herein, a novel glycoside hydrolase family 46 chitosanase (BaCsn46A) was cloned and expressed from Bacillus amyloliquefaciens. BaCsn46A exhibited good enzymatic properties with high specific activity (1031.2 U/mg) under optimal hydrolysis conditions of pH 6.0 and 50 degrees C. BaCsn46A is an endo-type chitosanase, which could be utilized to yield abundant chitooligosaccharides. Furthermore, the preparation of high degree of polymerization chitooligosaccharides (DP 5-10) was investigated by BaCsn46A in an enzymatic reactor with a subsequent series of membranes. The maximum concentration of total chitooligosaccharides (DP 2-10) was 28.11 g/L (93.70% yield). The proportion of high degree of polymerization chitooligosaccharides (DP 5-10) occupied 56.17% (w/w) of total chitooligosaccharides. This study provided a cleaner production process for the controllable preparation of chitooligosaccharides with specific degree of polymerization.