文献类型：期刊文献

英文题名：Combined available nitrogen resources enhanced erythromycin production and preliminary exploration of metabolic flux analysis under nitrogen perturbations

作者：Zhang, Qi[1];Hang, Haifeng[1];Tian, Xiwei[1];Zeng, Wei[2];Yu, Zhenhua[2];Wang, Xiaojian[2];Tang, Yin[1];Zhuang, Yingping[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Yidu HEC Biochem Co Ltd, Yidu 443300, Hubei, Peoples R China

年份：2019

卷号：42

期号：11

起止页码：1747

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20193207298946);WOS:【SCI-EXPANDED(收录号:WOS:000491946000004)】；

基金：This work was financially supported by the National Key Research and Development Program (2017YFB0309302), the National Basic Research Program of China (973 Program) (No. 2012CB721006), National Natural Science Foundation of China (No. 21276081) and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：Erythromycin; Available nitrogen sources; Corn steep liquor; Yeast powder; Metabolic flux

摘要：In the current study, the effect of different available nitrogen sources on erythromycin fermentation by Saccharopolyspora erythraea No. 8 is evaluated. Three different combinations of corn steep liquor and yeast powder were developed to investigate their impacts on erythromycin production. The results indicate that the optimal combination of available nitrogen sources was 10.0 g/L corn steep liquor and 4.0 g/L yeast power, generating a maximum yield of erythromycin of 13672 U/mL. To explore the effects of nitrogen perturbations on cell metabolism, metabolic flux analyses were performed and compared under different conditions. A high flux pentose phosphate pathway provided more NADPH for erythromycin synthesis via nitrogen optimization. Moreover, high n-propanol specific consumption rate enhanced erythromycin synthesis and n-propanol flowed into the central carbon metabolism by methylmalonyl-CoA node. These results indicate that the selection of an appropriate organic nitrogen source is essential for cell metabolism and erythromycin synthesis, and this is the first report of the successful application of available nitrogen source combinations in industrial erythromycin production.