文献类型：期刊文献

英文题名：Anti-Proliferation and Induced Mitochondria-Mediated Apoptosis of Ganoderic Acid Mk From Ganoderma lucidum Mycelia in Cervical Cancer HeLa Cells

作者：Liu, Ru-Ming[1];Li, Ying-Bo[1];Zhong, Jian-Jiang[1,2,3]

机构：[1]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Lab Mol Biochem Engn, Shanghai 200240, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2012

卷号：31

期号：1

起止页码：43

外文期刊名：LATIN AMERICAN JOURNAL OF PHARMACY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000304290800007)】；

基金：Financial support from National Natural Science Foundation of China (No. 30821005), the Shanghai Science & Technology Commission (project No. 054319933 and 08DZ1971900), and the Shanghai Leading Academic Discipline Project (project Nos. B203 and B505) is gratefully acknowledged.

语种：英文

外文关键词：Anti-proliferation; Apoptosis; Ganoderic acid Mk (GA-Mk); HeLa cells; Mitochondria

摘要：Ganoderic acid Mk (GA-Mk), a triterpenoid acid, was isolated from the mycelia of Ganoderma lucidum, and no biological activity of GA-Mk has ever been reported. In this work, we investigated the effect of GA-Mk on the cell proliferation and apoptosis in HeLa cells. The MTT results demonstrated that GA-Mk displayed interesting cytotoxicity toward various human cancer cell lines. Bromodeoxyuridine (BrdU) incorporation assay showed that GA-Mk had a dose-dependent inhibitory effect on proliferation of HeLa cells. The flow cytometry analysis indicated that the treatment of HeLa cells with GA-Mk increased the rate of early and late apoptotic cells in a dose-dependent manner. Furthermore, GA-Mk inducing apoptosis was in association with the burst of intracellular reactive oxygen species (ROS), the decrease of the mitochondrial membrane potential and the increase of caspase-3 and caspase-9 activities. These results demonstrated that GA-Mk was efficiently anti-proliferative and could induce apoptosis of HeLa cells by mitochondria-mediated pathway, and it may serve as a promising candidate for the treatment of cervical cancer.