文献类型：期刊文献

中文题名：多肽修饰牛血清白蛋白递送体系构建及其肝癌细胞靶向诊疗研究

英文题名：Construction of Peptide-Modified Bovine Serum Albumin Delivery System for the Targeted Diagnosis and Treatment of Liver Cancer Cells

作者：张智宁[1];王辰瀚[1];胡习乐[1];贺晓鹏[1]

机构：[1]华东理工大学结构可控先进功能材料及其制备教育部重点实验室,费林加诺贝尔奖科学家联合研究中心,上海200237

年份：2026

卷号：52

期号：2

起止页码：176

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金重大研究计划集成项目(92253306)。

语种：中文

中文关键词：肝细胞癌;GPC3;牛血清白蛋白;靶向递送;荧光成像

外文关键词：hepatocellular carcinoma;glypican-3;bovine serum albumin;targeted delivery;fluorescence imaging

摘要：肝细胞癌(HCC)在我国发病率较高且往往难以在早期发现,发展可特异性靶向示踪HCC细胞的诊断试剂有助于癌症的早期诊治。本文发展了一类新型磷脂酰肌醇蛋白聚糖-3(GPC3)靶向诊疗生物材料,利用牛血清白蛋白(BSA)为载体,通过简易的马来酰亚胺与蛋白质半胱氨酸残基进行Michael加成反应,在蛋白载体上修饰可靶向GPC3的多肽,进而通过主客体自组装将具有荧光性质和光动力治疗性能的二氢卟吩e6(Ce6)负载于多肽修饰的BSA蛋白中,以构建形成HCC特异靶向性的诊疗生物材料。研究结果表明,该诊疗材料对GPC3高表达HepG2细胞的荧光强度显著强于低表达细胞(p<0.001),且在630 nm光照下,生物材料处理的HepG2细胞存活率显著低于对照组人肝癌细胞(p<0.05),证实了生物材料的靶向诊疗效果。本文研究工作为HCC细胞的早期诊治提供了一类全新的靶向递送材料。
Hepatocellular carcinoma(HCC)has a high incidence in China and is often difficult to detect early.Developing diagnostic reagents that can specifically target and trace HCC cells is crucial for early diagnosis and treatment of cancer.In this study,a novel glypican-3(GPC3)-targeted theranostic biomaterial was developed.Using bovine serum albumin(BSA)as a carrier,GPC3-targeting peptides were modified onto the protein via a simple Michael addition reaction between maleimide and cysteine residues of the protein.Subsequently,chlorin e6(Ce6),a photosensitizer with fluorescence and photodynamic therapy properties,was loaded into the peptide-modified BSA through host-guest self-assembly,constructing a HCC-specific targeted theranostic biomaterial.The results showed that the theranostic material exhibited significantly stronger fluorescence intensity in GPC3-highly expressed HepG2 cells than in low-expression cells(p<0.001).Under 630 nm light irradiation,the viability of HepG2 cells treated with the biomaterial was significantly lower than that of control PLC(Primary Liver Carcinoma)cells at the same concentration(p<0.05),confirming the targeted theranostic effect of the biomaterial.This work provides a novel targeting delivery material for the early diagnosis and treatment of HCC cells.