文献类型：期刊文献

中文题名：水力停留时间和蛋白质对活性污泥毒性的影响

英文题名：Influence of Hydraulic Retention Time and Protein on Activated Sludge Toxicity

作者：李佳慧[1];陈秀荣[1];于泽亚[1];庄有军[1];周天俊[1];王晓晓[1]

机构：[1]华东理工大学资源与环境工程学院国家环境保护化工过程环境风险评价与控制重点实验室,上海200237

年份：2018

卷号：34

期号：15

起止页码：100

中文期刊名：中国给水排水

外文期刊名：China Water & Wastewater

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金资助项目(51008124)

语种：中文

中文关键词：污泥毒性;2;4;6-三氯酚;水力停留时间;蛋白质;序批式活性污泥法

外文关键词：sludge toxicity;2,4,6-TCP;HRT;protein;SBR

摘要：采用序批式活性污泥法(SBR)处理含2,4,6-三氯酚(2,4,6-TCP)的模拟废水,旨在研究水力停留时间(HRT)对污泥毒性的影响以及活性污泥分泌的蛋白质与毒性的关系。在不同HRT(8和12 h)条件下,分析活性污泥对2,4,6-TCP的去除效果及污泥毒性的变化,利用聚丙烯酰胺凝胶电泳(SDS-PAGE)技术对污泥中的蛋白质进行分离,并利用基质辅助激光解析电离飞行时间质谱法(MALDI-TOF/TOF MS)对降解2,4,6-TCP的活性污泥和空白组中有显著差异的蛋白条带进行鉴定。结果表明,HRT为8 h时污泥毒性较低,且污泥毒性降到最低并趋于稳定所需的时间比12 h的提前了10 d。降解2,4,6-TCP的活性污泥与空白组相比,胞外热应激蛋白和外膜蛋白的过量表达与污泥毒性密切相关,可以增强污泥中微生物抵抗毒性物质的能力。
In order to investigate the influence of hydraulic retention time （HRT） on sludge toxicity and the relationship between proteins secretion by activated sludge and sludge toxicity, sequence batch reactor （SBR） was used for biological treatment of simulated wastewater containing 2,4,6-trichlorophenol （2,4,6-TCP）. Under the condition of different HRT （8 h and 12 h）, the removal efficiency of 2,4,6- TCP and the variation of sludge toxicity were analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） was used to separate proteins in activated sludge, and the significant differential protein bands in activated sludge degrading 2,4, 6-TCP and blank group were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry （ MALDI-TOF/TOF MS ）. Results showed that sludge toxicity was lower when HRT was 8 h, and the time required for sludge toxicity reduced to the minimum and tended to be stable pared with the blank group, overexpression of was l0 days earlier than that when HRT was 12 h. Comextracellular heat shock protein （HSP） and outer merebrane protein of the activated sludge degraded 2,4,6-TCP were closely related to the sludge toxicity, which could enhance the ability of microorganisms in activated sludge to resist toxic substances.