文献类型：期刊文献

英文题名：An AIE-active fluorescent probe with near-infrared (NIR) emission for selectively monitoring and bioimaging Cys in living cells

作者：Zhang, Zhen[1,2];Zhao, Weijun[1,2];Chen, Yun[1,2];Wei, Sheng-Hao[1,2];Zhu, Fei[1,2];Zhu, Xu-Zhong[1,2];Wang, Cheng-Yun[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：140

外文期刊名：TETRAHEDRON

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001024653500001)】；

基金：The authors are grateful to financial support from Natural Sci- ence Foundation of Shanghai (No. 22ZR1417300) .

语种：英文

外文关键词：Fluorescent probe; Aggregation-induced emission (AIE); Cysteine (Cys); Near-infrared (NIR) emission; Bioimaging

摘要：Cysteine (Cys), as a kind of essential bio-thiols, plays a pivotal role in modulating cell metabolism, maintaining intracellular redox balance and facilitating protein synthesis. In order to investigate its role in these physiological processes, fluorescent probe was selected as an important method for selectively detecting Cys. However, most of the probes possess ACQ effect, which limits their applications in the field of biosensing and bioimaging. Herein, an AIE-active fluorescent probe TCF-TPA was synthesized for specifically detecting Cys in HeLa cells. TCF-TPA, consisted of a tricyanofuran (TCF) fluorophore, was modified with an acrylate group for distinguish Cys from other bio-thiols, and the incorporation of electron-donating triphenylamine endowed the probe with AIE property and strong intramolecular charge transfer effect, thereby enabling the probe to have near-infrared (NIR) emission length (666 nm). Meanwhile, TCF-TPA possessed low detection limit for Cys (73.0 nM) and was successfully performed bioimaging of Cys in HeLa cells. & COPY; 2023 Elsevier Ltd. All rights reserved.