文献类型：期刊文献

英文题名：Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

作者：Chen, Tiantian[1];Yin, Liyan[1];Huang, Chusen[1,2,3];Qin, Yiqiao[1];Zhu, Weiping[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Key Lab Resource Chem,Educ Minist, Shanghai 200234, Peoples R China;[3]Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai Key Lab Rare Earth Funct Mat, Shanghai 200234, Peoples R China

年份：2015

卷号：66

起止页码：259

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20144800266352);WOS:【SCI-EXPANDED(收录号:WOS:000348619800038)】；

基金：We thank the financial support from the National Science and Technology Pillar Program of China (2011BAE06B02), National Basic Research Program of China (973 Program, 2013CB733700), National Natural Science Foundation of China (21476077, 21236002), Shanghai Pujiang Program and the Fundamental Research Funds for the Central Universities (WK1013002).

语种：英文

外文关键词：Naphthalimide; Fluorescent probe; Copper ion; Histidine; Live cell imaging

摘要：A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu2+) in HEPES buffer (50 mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu2+ ranging from 0-8.3 mu M (correlation coefficient R-2=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200 mu M (correlation coefficient R-2=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity. (C) 2014 Elsevier B.V. All rights reserved.