文献类型：期刊文献

英文题名：The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana

作者：Wan, Minxi[1,2,3];Jin, Xuejie[2];Xia, Jinlan[2];Rosenberg, Julian N.[3];Yu, Geng[3];Nie, Zhenyuan[2];Oyler, George A.[4];Betenbaugh, Michael J.[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China;[3]Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA;[4]Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA

年份：2014

卷号：98

期号：22

起止页码：9473

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20144500177628);WOS:【SCI-EXPANDED(收录号:WOS:000344344500027)】；

基金：This work was supported by the Chinese Natural Science Foundation For Distinguished Group (No. 50621063), Graduate Innovation Project of Central South University (No. 2010bsxt05), and NSF EFRI Grant (No. 1332344).

语种：英文

外文关键词：Chlorella sorokiniana; Fe; Lipid biosynthesis; qPCR; Genefishing

摘要：The effects of iron on the growth, lipid accumulation, and gene expression profiles of the limnetic Chlorella sorokiniana CCTCC M209220 under photoautotrophy were investigated. The addition of iron up to 10(-5) mol l(-l) increased final cell densities by nearly 2-fold at 2.3 x 10(7) cells/ml, growth rate by 2-fold, and the length of the exponential phase by 5 days as compared to unsupplemented controls while 10(-3) mol l(-1) iron was toxic. The lipid content increased from 12 % for unsupplemented cultures to 33 % at 10(-4) mol l(-1) iron while the highest overall lipid yield reached 179 mg l(-1). A genefishing and qPCR comparison between the C. sorokiniana at low and high iron levels indicated increases in the expression of several genes, including carbonic anhydrase involved in microalgal cell growth, as well as acc1 and choline transporter related to lipid synthesis. This study provides insights into changes in gene expression and metabolism that accompany iron supplementation to Chlorella as well as potential metabolic engineering targets for improving growth and lipid synthesis in microalgae.