文献类型：期刊文献

英文题名：Identifying sesterterpenoids via feature-based molecular networking and small-scale fermentation

作者：Lv, Kangjie[1];Duan, Yuyang[1];Li, Xiaoying[1];Wang, Xinye[1];Xing, Cuiping[1];Lan, Keying[1];Zhu, Bin[2];Zhu, Guoliang[1];Qiu, Yuyang[3];Li, Songwei[4];Hsiang, Tom[5];Zhang, Lixin[1];Jiang, Lan[6];Liu, Xueting[1,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Lab Pharmaceut Crystal Engn Res & Technol, Shanghai 200237, Peoples R China;[3]Shandong Univ Finance & Econ, Sch Insurance, Jinan 250014, Peoples R China;[4]Shanghai Univ, Sch Med, Shanghai 200444, Peoples R China;[5]Univ Guelph, Sch Environm Sci, 50 Stone Rd East, Guelph, ON N1G 2W1, Canada;[6]Nanjing Med Univ, Dept Cardiothorac Surg, Childrens Hosp, Nanjing 210093, Peoples R China

年份：2024

卷号：108

期号：1

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20244217188511);WOS:【SCI-EXPANDED(收录号:WOS:001331299800001)】；

基金：This work was supported by the National Natural Science Foundation of China (21977029, 21907031) to XL and LJ and the National Key Research and Development Program of China (2019YFA0906200 and 2020YFA090032) to XL and LZ. We are grateful for the funding support from The Science and Technology Commission of Shanghai Municipality (21NL2600100) to XL. Furthermore, we would like to acknowledge the Natural Science and Engineering Research Council of Canada for funding the genome sequencing and assembly of F. oxysporum strain FO14005 by TH.

语种：英文

外文关键词：Bifunctional terpene synthases; Feature-based molecular networking; Cytochrome P450; Aspergillus oryzae; Heterologous expression

摘要：Terpenoids are known for their diverse structures and broad bioactivities with significant potential in pharmaceutical applications. However, natural products with low yields are usually ignored in traditional chemical analysis. Feature-based molecular networking (FBMN) was developed recently to cluster compounds with similar skeletons, which can highlight trace amounts of unknown compounds. Fusoxypene A is a sesterterpene synthesized by Fusarium oxysporum fusoxypene synthase (FoFS) with a unique 5/6/7/3/5 ring system. In this study, the FoFS-containing biosynthetic gene cluster was identified from F. oxysporum FO14005, and an efficient FBMN-based strategy was established to characterize four new sesterterpenoids, fusoxyordienoid A-D (1-4), based on a small-scale fermentation strategy. A cytochrome P450 monooxygenase, FusB, was found to be involved in the functionalization of fusoxypene A at C-17 and C-24 and responsible for the hydroxylation of fusoxyordienoid A at C-1 and C-8. This study highlights the potential of FBMN as a powerful tool for the discovery and characterization of natural compounds with low abundance.