文献类型：期刊文献

英文题名：Decellularized Extracellular Matrix Enriched With Gdnf Enhancing Neurogenesis and Remyelination For Improving Motor Recovery after Spinal Cord Injury

作者：Liu, Jiashang[1]; Yan, Ruijia[1]; Wang, Bixue[1]; Chen, Shu[1]; Hong, Hua[1]; Liu, Changsheng[1]; Chen, Xi[1]

机构：[1] Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Engineering Research Center for Biomaterials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230362110)

语种：英文

外文关键词：Cell culture - Cell signaling - Flowcharting - Molecular biology - Neurons - Patient rehabilitation - Recovery - Stem cells

摘要：Motor functional improvement represents a paramount treatment objective in the post-spinal cord injury (SCI) recovery process. However, neuronal cell death and axonal degeneration following SCI disrupt neural signaling, impeding the achievement of motor functional recovery. In this study, we developed a novel multifunctional decellularized extracellular matrix derived from spinal cord tissue (dSECM), crosslinked with glial cell-derived neurotrophic factor (GDNF), to promote differentiation of mesenchymal stem cells (MSCs) into neurons and facilitate axonogenesis and remyelination. After decellularization, the immunogenic cellular components were effectively removed in dSECM, while the crucial protein components were preserved which supports MSCs proliferation and differentiation. Furthermore, sustained release of GDNF from the dSECM facilitated axonogenesis and remyelination by activating the PI3K/Akt and MEK/Erk pathways. Our findings demonstrate that the dSECM-GDNF platform promoted neurogenesis, axonogenesis, and remyelination to enhance neural signaling, thereby yielding promising therapeutic effects for motor functional improvement after SCI. ? 2023, The Authors. All rights reserved.