文献类型：期刊文献

英文题名：An acetal-based polymeric crosslinker with controlled pH-sensitivity

作者：Cao, Hongliang[1,2];Dong, Yixiao[3];Bre, Ligia[3];Tapeinos, Christos[4];Wang, Wenxin[3];Pandit, Abhay[4]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Adv Polymer Mat, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Univ Coll Dublin, Charles Inst Dermatol, Dublin, Ireland;[4]Natl Univ Ireland, Ctr Res Med Devices, Galway, Ireland

年份：2016

卷号：6

期号：12

起止页码：9604

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20160501880759);WOS:【SCI-EXPANDED(收录号:WOS:000369516100029)】；

基金：We gratefully acknowledge the financial support of Shanghai Pujiang Program (13PJ1401700) and the European Commission under the DISC REGENERATION project (NMP3-LA-2008-213904).

语种：英文

外文关键词：Atom transfer radical polymerization - Hydrogels - Polyethylene oxides - Polyethylene glycols - Medical applications - Mechanical stability

摘要：Even though collagen hydrogels have been used in many biomedical applications due to their unique characteristics, they lack in mechanical stability and resistance to enzymatic degradation. Aiming at the elimination of these drawbacks, collagen hydrogels have been crosslinked with a variety of synthetic crosslinkers all of which presented increased toxicity, which resulted in their limited use in biomedical devices. In order to overcome this limitation, a branched copolymer based on N-hydroxysuccinimide (NHS) activated poly(ethylene glycol) (PEG) groups was synthesized by in situ deactivation enhanced atom transfer radical polymerization (DE-ATRP) and used as crosslinker for collagen hydrogels. The NHS-PEG polymer was modified with labile acetal linkages (acetal-NHS) which made it responsive to pH-alterations and its responsiveness was studied at various pH. The activated acetal-NHS polymeric crosslinker demonstrated long-term stability at neutral pH but underwent hydrolysis at acidic pH, showing strongly pH-dependent degradation properties. By using the acetal-NHS polymeric crosslinker, collagen hydrogels with controlled degradation profiles were fabricated. Both the acetal-NHS crosslinker and the crosslinked collagen hydrogels exhibited excellent cytocompatibility.