文献类型：期刊文献

英文题名：Galactosylated poly(ε-caprolactone) membrane promoted liver-specific functions of HepG2 cells in vitro

作者：Zhang, Yan[1];Zhang, Yi[1];Chen, Min[2];Zhou, Yan[2];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：41

起止页码：52

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20142017712907);WOS:【SCI-EXPANDED(收录号:WOS:000338606700007)】；

基金：Financial support from the National Natural Science Foundation of China (20804015, 21274039) and Basic Research Key Program Project of the Commission of Science and Technology of Shanghai (12JC1403000, 12JC1403100) are gratefully acknowledged.

语种：英文

外文关键词：Poly(epsilon-caprolactone); Galactose; HepG2 cells; Hepatic tissue engineering; Modified surface

摘要：The lack of pendant functional groups on the PCL backbone has been a great challenge for surface bioactivation of poly(epsilon-caprolactone) (PCL). In the present study, covalently galactosylated PCL (GPCL) was developed through coupling between the amino-functionalized PCL (NPCL) and the lactobionic acid (LA) and its potential application in maintenance of physiological functions of HepG2 cells was further evaluated. The structure and properties of GPCL were explored by H-1 NMR, FT-IR, GPC and DSC Moreover, the incorporation of galactose ligands onto GPCL membranes not only promoted higher wettability, but also radically changed surface morphology in comparison with PCL and NPCL according to the contact angle measurement and atomic force microscopy. When HepG2 cells were seeded onto these membranes, the cells on GPCL membranes showed more pronounced cell adhesion and tended to form aggregates during the initial adhesion stage and then progressively grew into multi-layer structures compared to those without galactose ligands by the observation with fluorescence microscope and scanning electron microscopy. Furthermore, live dead assay and functional tests demonstrated that HepG2 cells on GPCL membranes had superior viability and maintained better liver-specific functions. Collectively, GPCL has great potential for hepatic tissue engineering scaffolds. (C) 2014 Elsevier B.V. All rights reserved.