文献类型：期刊文献

英文题名：Nuclear localization signal of ING4 plays a key role in its binding to p53

作者：Zhang, X; Wang, KS; Wang, ZQ; Xu, LS; Wang, QW; Chen, F; Wei, DZ; Han, ZG

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Natl Human Genome Ctr, Shanghai 201203, Peoples R China

年份：2005

卷号：331

期号：4

起止页码：1032

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000229234500021)】；

语种：英文

外文关键词：ING4; p53; nuclear localization signal; protein-protein interaction; subcellular localization; transcriptional activity; p21

摘要：ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53 and negatively regulate the cell growth with significant G2/M arrest of cell cycle in HepG2 cells through upregulation of p53-inducible gene p21. However, which region of ING4 could have contributed to the binding to p53 remains largely unclear. Herein, the GST-pulldown experiments revealed that the middle region of ING4, a potential bipartite nuclear localization signal (NLS), could be involved in the binding to p53. Furthermore, the interaction of ING4 to p53 was abrogated in vitro and in vivo when certain mutations or the entire deletion of the NLS domain occurred. More interestingly, the mutations of the NLS domain could alter the ING4 nuclear localization, disrupt the interaction of ING4 with p53, and even, deregulate the p53-inducible gene p21 in MCF-7 cells. All data indicated that the NLS domain of ING4 is essential for the binding of ING4 to p53 and the function of ING4 associated with p53. (c) 2005 Elsevier Inc. All rights reserved.