文献类型：期刊文献

中文题名：酶法合成抗病毒药物阿糖腺苷

英文题名：Enzymatic Synthesis of Antivirus Agent Adenine Arabinoside

作者：郭勇莉[1];丁庆豹[1];欧伶[1];邱蔚然[2]

机构：[1]华东理工大学生物反应器工程重点实验室,上海200237;[2]上海秋之友生物科技有限公司,上海205169

年份：2006

卷号：16

期号：1

起止页码：32

中文期刊名：生物技术

外文期刊名：Biotechnology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：产气肠杆菌;阿糖尿苷;阿糖腺苷;尿苷磷酸化酶;嘌呤核苷磷酸化酶

外文关键词：nucleoside phosphorylase Enterobacter aerogenes ; arabinofuranosyluracil; adenine arabinoside ； uridine phosphorylase; purine

摘要：目的:为了开发一种生产阿糖腺苷的有效方法。方法:研究了以产气肠杆菌完整细胞为催化剂酶法合成阿糖腺苷,优化了菌体培养条件以及酶反应条件。结果:在培养基中添加0.5%葡萄糖,33℃下培养16h,既能得到较多菌体,又能使菌体的催化活性保持较高。酶反应在pH7.0、25mmol/L的磷酸钾缓冲液中进行,底物浓度为阿糖尿苷30mmol/L,腺嘌呤10mmol/L,加入10%湿菌体,在60℃下振荡反应48h,腺嘌呤转化率可达90%。结论:酶法合成阿糖腺苷可应用于大规模工业化生产。
Objective：In order to establish an effective process to produce adenine arabinoside （ara- A）, enzymatic synthesis ot ara - A catalyzed by whole cells of Enterobacter aerogenes was studied. Methods：The conditions of culture and enzymatic reaction were optimized. Results： Whole ceils of Enterobacter aerogenes, cultured at 33℃ in medium containing 0.5% glucose, incubated for 16h, demonstrated relatively high activity, and under this culture condition more wet cells could be harvested. 90% of adenine could be transformed into ara - A after 48h enzymatic reaction at 60℃ in 25mmol/L phosphate buffer（pHT.0） with 10% wet cells of Enterobacter aerogenes, which contained 30mmol/L ara- U and 10mmol/L adenine. Conclusion：The result indicated that enzymatic synthesis of ara - A was practicable-to be applied to industrial production.