文献类型：期刊文献

英文题名：Detection of total protein in milk using phosphomolybdic acid-mediated surface-enhanced Raman spectroscopy

作者：Huang, Yingzhao[1,2];Gu, Jianming[2];Xiang, Gang[1];Xu, Jiajie[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ, Sch Life Sci, 99 Shangda Rd, Shanghai 200444, Peoples R China

年份：2016

卷号：47

期号：3

起止页码：277

外文期刊名：JOURNAL OF RAMAN SPECTROSCOPY

收录：;EI(收录号：20231113720188);WOS:【SCI-EXPANDED(收录号:WOS:000372322800003)】；

基金：This work was supported by the National Natural Science Foundation of China under grant no. 31271862.

语种：英文

外文关键词：total protein; casein; milk; phosphomolybdic acid; SERS

摘要：Although many surface-enhanced Raman scattering (SERS)-based methods for detecting specific proteins have been studied, simple and direct detection of total protein in liquid using a SERS-based method remains difficult. In this study, a distinguishable effect on the SERS spectra from pre-mixture of phosphomolybdic acid (PMA) with protein was found, indicating that PMA could be used as a SERS reporter for total protein detection in a liquid sample. Further experiments confirmed a good linear relationship between a premixed concentration of protein (casein, whey protein or bovine serum albumin) and the SERS intensity of PMA in our SERS system. Using casein as a reference, a PMA-mediated SERS method was proposed that can quantitatively analyze protein at 2.5-25 mu g/ml with a limit of detection of 1.5 mu g/ml. Our PMA-mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk. Copyright (c) 2015 John Wiley & Sons, Ltd.