文献类型：期刊文献

英文题名：Bent-to-planar Si-rhodamines: a distinct rehybridization lights up NIR-II fluorescence for tracking nitric oxide in the Alzheimer's disease brain

作者：Xu, Qingshuang[1];Zhang, Yutao[1];Zhu, Mingming[2];Yan, Chenxu[1];Mao, Wenle[1];Zhu, Wei-Hong[1];Guo, Zhiqian[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat & Joint Int Re, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Inst Digest Dis, Inflammatory Bowel Dis Res Ctr,Div Gastroenterol &, Shanghai, Peoples R China

年份：2023

卷号：14

期号：15

起止页码：4091

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20231513867693);WOS:【SCI-EXPANDED(收录号:WOS:000956353400001)】；

基金：This work was supported by NSFC/China (22225805 and 32121005), National Key Research and Development Program of China (2021YFA0910000), Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03, 21JC1401700), Innovation Program of Shanghai Municipal Education Commission, Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), and Programme of Introducing Talents of Discipline to Universities (B16017), and sponsored by Shanghai Pujiang Program (21PJD038). We thank Weimin Liu, ShanghaiTech University, for the femtosecond time-resolved transient absorption spectroscopy experimental support.

语种：英文

外文关键词：Clinical research - Fluorescence - Infrared devices - Mammals - Neurodegenerative diseases - Nitric oxide - Silicon - Silicon compounds

摘要：An ongoing revolution in fluorescence-based technologies has transformed the way we visualize and manipulate biological events. An enduring goal in this field is to explore high-performance fluorogenic scaffolds that show tunability and capability for in vivo analysis, especially for small-molecular near-infrared (NIR) fluorophores. We present a unique bent-to-planar rehybridization design strategy for NIR fluorogenic scaffolds, thus yielding a palette of switchable bent/planar Si-rhodamines that span from visible to NIR-II wavelengths. We demonstrate that the rehybridization of meso-nitrogen in this innovative NIR scaffold Cl-SiRhd results in flipping between the disruption and recovery of the polymethine pi-electron system, thereby significantly altering the spectral wavelength with crosstalk-free responses. Using elaborately lighting-up NIR-II probes with ultra-large Stokes shifts (ca. 250 nm), we successfully achieve real-time in situ monitoring of biological events in live cells, zebrafish, and mice. Notably, for the first time, the light-up NIR-II probe makes a breakthrough in directly in situ tracking nitric oxide (NO) fluctuations in the brains of mice with Alzheimer's disease. This de novo bent-to-planar rehybridization strategy of NIR-II probes opens up exciting opportunities for expanding the in vivo imaging toolbox in both life science research and clinical applications.