文献类型：期刊文献

英文题名：2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone Promoted Glucose Uptake and Imposed a Paradoxical Effect on Adipocyte Differentiation in 3T3-L1 Cells

作者：Hu, Ying-Chun[1,2];Zhang, Zhe[2];Shi, Wei-Gang[3];Mi, Ting-Yan[2];Zhou, Lu-Xian[2];Huang, Nan[2];Hoptroff, Michael[2];Lu, Yan-Hua[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Unilever R&D Shanghai, Shanghai 200335, Peoples R China;[3]Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China

年份：2014

卷号：62

期号：8

起止页码：1898

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20141017428879);WOS:【SCI-EXPANDED(收录号:WOS:000332144400021)】；

基金：This work was supported by The Fundamental Research Funds for the Central Universities and partially supported by Shanghai Leading Academic Discipline Project (B505) and the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：2 ',4 '-dihydroxy-6 '-methoxy-3 ',5 '-dimethylchalcone; 3T3-L1 adipocytes; glucose uptake; lipid accumulation; PPAR-gamma

摘要：2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), one of the flavonoids isolated and purified from the dried flower buds of Cleistocalyx operculatus, was explored for its function in glucose uptake/glycogen synthesis in insulin-sensitive tissue cells and its effect and mechanism on 3T3-L1 preadipocyte differentiation. DMC (10 mu M) treatment remarkably promoted glucose uptake in differentiated 3T3-L1 adipocytes (P < 0.05 vs control group), whereas the glucose uptake in L6 myoblasts and glycogen synthesis in HepG2 hepatocytes were not affected by the treatment. DMC had paradoxical effects on lipid accumulation in 3T3-L1 cells compared with differentiation control. High concentrations of DMC (10 and 20 mu M) markedly diminished lipid accumulation; however, a low concentration of DMC (2.5 mu M) enhanced lipid storage in 3T3-L1 cells (P < 0.01 vs differentiation control group), and 5 mu M DMC did not impose a significant effect. It was demonstrated that the effect of DMC in lipid accumulation was controlled by the expression of PPAR-gamma.