文献类型：期刊文献

英文题名：Overexpression of glutathione synthetase gene improving redox homeostasis and chicken infectious bursal disease virus propagation in chicken embryo fibroblast DF-1

作者：Lin, Jia[1,2];Min, Rui[1];Yi, Xiaoping[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Fujian Univ Tradit Chinese Med, Acad Rehabil Ind, Collaborat Innovat Ctr Rehabil Technol, Fuzhou, Peoples R China

年份：2023

卷号：10

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20233714717433);WOS:【SCI-EXPANDED(收录号:WOS:001063780700001)】；

基金：The authors thank Prof. Yingping Zhuang and Prof. Xiaoping Yi for guidance.

语种：英文

外文关键词：Chicken infectious bursal virus; Chicken embryonic fibroblasts DF-1; Glutathione; Glutathione synthetase

摘要：Infectious bursal disease (IBD) of chickens is an acute, high-contact, lytic infectious disease caused by infectious bursal disease virus (IBDV). The attenuated inactivated vaccine produced by DF-1 cells is an effective control method, but the epidemic protection demands from the world poultry industry remain unfulfilled. To improve the IBDV vaccine production capacity and reduce the economic losses caused by IBDV in chicken, cellular metabolic engineering is performed on host cells. In this study, when analyzing the metabolomic after IBDV infection of DF-1 cells and the exogenous addition of reduced glutathione (GSH), we found that glutathione metabolism had an important role in the propagation of IBDV in DF-1 cells, and the glutathione synthetase gene (gss) could be a limiting regulator in glutathione metabolism. Therefore, three stable recombinant cell lines GSS-L, GSS-M, and GSS-H (gss gene overexpression with low, medium, and high mRNA levels) were screened. We found that the recombinant GSS-M cell line had the optimal regulatory effect with a 7.19 & PLUSMN; 0.93-fold increase in IBDV titer. We performed oxidative stress and redox status analysis on different recombinant cell lines, and found that the overexpression of gss gene significantly enhanced the ability of host cells to resist oxidative stress caused by IBDV infection. This study established a high-efficiency DF-1 cells system for IBDV vaccine production by regulating glutathione metabolism, and underscored the importance of moderate gene expression regulation on the virus reproduction providing a way for rational and precise cell engineering.